ROS production in homogenate from the body wall of sea cucumber Stichopus japonicus under UVA irradiation: ESR spin-trapping study. (1st February 2016)

• Record Type: Journal Article
• Authors: Qi, Hang; Dong, Xiu-fang; Zhao, Ya-ping; Li, Nan; Fu, Hui; Feng, Ding-ding; Liu, Li; Yu, Chen-xu
• Is Part Of: Food chemistry. Volume 192(2016)
• Page Start: 358
• Page End: 362
• Publication Date: 2016-02-01
• Subjects: Sea cucumber Stichopus japonicus -- Homogenate -- DMPO -- Hydroxyl radicals -- UVA irradiation; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.07.030
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: OH was produced in the homogenate from S. japonicus under UVA irradiation. There was one of ways as follows: O2 − → H2 O2 → OH . OH might be a critical factor in UVA-induced S. japonicus autolysis. It provided a new basis for being rich in mechanism of S. japonicus autolysis. Abstract: Sea cucumber Stichopus japonicus ( S. japonicus ) shows a strong ability of autolysis, which leads to severe deterioration in sea cucumber quality during processing and storage. In this study, to further characterize the mechanism of sea cucumber autolysis, hydroxyl radical production induced by ultraviolet A (UVA) irradiation was investigated. Homogenate from the body wall of S. japonicas was prepared and subjected to UVA irradiation at room temperature. Electron Spin Resonance (ESR) spectra of the treated samples were subsequently recorded. The results showed that hydroxyl radicals (OH ) became more abundant while the time of UVA treatment and the homogenate concentration were increased. Addition of superoxide dismutase (SOD), catalase, EDTA, desferal, NaN3 and D2 O to the homogenate samples led to different degrees of inhibition on OH production. Metal cations and pH also showed different effects on OH production. These results indicated that OH was produced in the homogenate with a possible pathway as follows: O2 − → H2 O2 → OH, suggesting that OH might be a critical factor in UVA-induced S. japonicus autolysis.