The development of a hexaplex-conventional PCR for identification of six animal and plant species in foodstuffs. (1st February 2016)

• Record Type: Journal Article
• Authors: Safdar, Muhammad; Junejo, Yasmeen
• Is Part Of: Food chemistry. Volume 192(2016)
• Page Start: 745
• Page End: 749
• Publication Date: 2016-02-01
• Subjects: Foodstuffs -- Species-specific primers -- Hexaplex-conventional PCR -- Mitochondrial DNA -- 18S rRNA -- Species origins identification; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2015.07.082
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Hexaplex PCR assays to identify six animal and plant species in a tube. The assays are specific, sensitive and reproducible. The practical approach is much easier, cheaper and faster. Successfully detection of food allergens and low price meats. Abstract: A hexaplex-conventional PCR assay was developed for identification of five meat and one plant species origins in foodstuffs simultaneously. The method merges the use of horse ( Equus caballus ), soybean ( Glycine max ), sheep ( Ovis aries ), poultry ( Meleagris meleagris ), pork ( Sus scrofa ), and cow ( Bos taurus ) specific primers that amplify fragments (horse; 85 bp, soybean; 100 bp, sheep; 119 bp, poultry; 183 bp, pork; 212 bp and cow; 271 bp) of the mitochondrial cyt b, lectin, 12S rRNA, 12S rRNA, ATPase subunit 6 genes and ATPase subunit 8 genes respectively, and a universal 18S rRNA primers that amplifies a 141 bp. Multiplex analysis of the reference food samples showed that detection limit of the hexaplex assay was 0.01% for each species. Taken together, all data indicated that this hexaplex PCR assay was a simple, fast, sensitive, specific, and cost-effective detection method for horse, soybean, sheep, poultry, pork and cow species in foodstuffs.