The influence of amylose content on the modification of starches by glycogen branching enzymes. (1st November 2022)

• Record Type: Journal Article
• Authors: Gaenssle, Aline L.O.; van der Maarel, Marc J.E.C.; Jurak, Edita
• Is Part Of: Food chemistry. Volume 393(2022)
• Publication Date: 2022-11-01
• Subjects: Glycogen branching enzyme -- Starch -- Maltodextrin -- Chain length distribution -- Molecular size; BfGBE GBE from Butyrivibrio fibrisolvens -- DP degree of polymerization -- GBE glycogen branching enzyme -- GH13 glycoside hydrolase family 13 -- GH57 glycoside hydrolase family 57 -- kDa kilo Daltons (1 kDa = 1 kg/mol) -- MD-Mix maltodextrin mixture of DP 10–40 -- PmGBE1 GBE1 from Petrotoga mobilis -- RmGBE GBE from Rhodothermus marinus -- SAF/LF ratio ratio between the accumulated peak areas of the chain fractions DP 9–12 (short fraction, SAF) and DP ≥ 13 (long fraction, LF) -- TkGBE GBE from Thermococcus kodakarensis -- TtGBE GBE from Thermus thermophilus; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133294
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Glycogen branching enzymes produce similar products from different substrates. GH13 GBEs generate smaller products than GH57 GBEs, even on a maltodextrin mixture. The substrate structure primarily influences in the amount of product obtained. Abstract: Glycogen branching enzymes (GBEs) have been used to generate new branches in starches for producing slowly digestible starches. The aim of this study was to expand the knowledge about the mode of action of these enzymes by identifying structural aspects of starchy substrates affecting the products generated by different GBEs. The structures obtained from incubating five GBEs (three from glycoside hydrolase family (GH) 13 and two from GH57) on five different substrates exhibited minor but statistically significant correlations between the amount of longer chains (degree of polymerization (DP) 9–24) of the product and both the amylose content and the degree of branching of the substrate (Pearson correlation coefficient of ≤−0.773 and ≥0.786, respectively). GH57 GBEs mainly generated large products with long branches (100–700 kDa and DP 11–16) whereas GH13 GBEs produced smaller products with shorter branches (6–150 kDa and DP 3–10).