Comprehensive lipidomic analysis of milk polar lipids using ultraperformance supercritical fluid chromatography-mass spectrometry. (1st November 2022)

• Record Type: Journal Article
• Authors: Jiang, Chenyu; Zhang, Xinghe; Yu, Jiahui; Yuan, Tinglan; Zhao, Pu; Tao, Guanjun; Wei, Wei; Wang, Xingguo
• Is Part Of: Food chemistry. Volume 393(2022)
• Publication Date: 2022-11-01
• Subjects: Polar lipid -- Human milk -- Ultraperformance supercritical fluid chromatography -- Membrane lipids -- Sphingomyelin; TAG triacylglycerol -- MFGM milk fat globule membrane -- PG phosphatidylglycerol -- PE phosphatidylethanolamine -- PI phosphatidylinositol -- CL cardiolipin -- LPE lysophosphatidylethanolamine -- PA phosphatidic acid -- PS phosphatidylserine -- PC phosphatidylcholine -- SM sphingomyelin -- LPC lysophosphatidylcholine -- LC liquid chromatography -- MS mass spectrometry -- UHPLC ultrahigh performance liquid chromatography -- HILIC hydrophilic liquid chromatography -- SPE solid phase extraction -- SFC supercritical fluid chromatography -- GC gas chromatography -- ESI electrospray ionization -- UPSFC-Q-TOF-MS ultraperformance supercritical fluid chromatography-quadrupole-time of flight-mass spectrometry -- DAG diacylglycerol -- Cer ceramide -- MAG monoacylglycerol -- TLC thin layer chromatography -- FFA free fatty acid -- DHA docosahexaenoic acid -- ARA arachidonic acid -- SFA saturated fatty acid -- MUFA monounsaturated fatty acid -- PUFA polyunsaturated fatty acid; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133336
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: We present an analytical approach for milk polar lipid analysis using UPSFC-MS. A total of 18 lipid classes were separated and analyzed within 10 min. 219 lipid molecular species were identified in milk and milk-derived samples. Polar lipids composition in human milk is obviously different from that in other milk. Saturation of the acyl groups of different polar lipids in human milk varies considerably. Abstract: Polar lipids in milk are receiving increasing interest due to their bioactivities. However, milk polar lipids present a wide range of physical–chemical properties at different concentrations, making their analysis challenging. In this study, we presented a comprehensive lipidomic method using ultraperformance supercritical fluid chromatography (UPSFC)-quadrupole-time of flight-mass spectrometry (Q-TOF-MS), which enabled the separation of 18 lipid classes (including nonpolar lipids, cholesterol, ceramide, glycerophospholipids, sphingomyelin, and gangliosides) within 10 min. The method was used to analyze the polar lipids in seven samples, including human milk, other mammalian milk and milk fat globule membrane ingredients, identifying 14 lipid classes containing 219 lipid molecular species. A mass spectrometry data processing strategy applicable for high-throughput studies was also developed and validated.