Phosphatase-like activity of single-atom CeNC nanozyme for rapid detection of Al3+. (1st October 2022)

• Record Type: Journal Article
• Authors: Song, Guangchun; Li, Jin-Cheng; Majid, Zainabu; Xu, Wentao; He, Xiaoyun; Yao, Zhiyi; Luo, Yunbo; Huang, Kunlun; Cheng, Nan
• Is Part Of: Food chemistry. Volume 390(2022)
• Publication Date: 2022-10-01
• Subjects: Phosphatase-like -- Single atom CeNC nanozyme -- Al3+ detection -- Food safety; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133127
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: An excellent PPA-like activity of SACeNC nanozyme other than oxidoreductase was synthesized. The SACeNC nanozyme with PPA-like activity has higher stability than commercial ALP. Al 3+ can specificity inhibited the PPA-like activity. A fast, portable, and efficient fluorescent liquid phase sensor to detect Al 3+ was constructed. Abstract: Single-atom nanozymes are a class of nanozymes with attractive enzyme-like activities. They usually mimic oxidoreductases and lack other types of enzyme-like activities. Hence, we verified a single-atom CeNC (SACeNC) nanozyme with an excellent phosphatase-like (PPA-like) activity, which could catalyze the dephosphorylation of inorganic phosphates. Meanwhile, we found that Al 3+ could specifically combine with the O atom in its structure to form an Al-O bond, which could inhibit its PPA-like activity. Based on this principle, we have constructed a fast, portable, and efficient fluorescent liquid phase sensor to detect Al 3+ . The detection time was only 4 min, and the limit of detection (LOD) was 22.89 ng/mL in the linear range of 5–25 μg/mL. This study not only verified that single-atom nanozymes mimic phosphatase activities, but also applied its unique enzyme-like to the field of food safety rapid detection.