A designed self-microemulsion delivery system for dihydromyricetin and its dietary intervention effect on high-fat-diet fed mice. (1st October 2022)

• Record Type: Journal Article
• Authors: Lyu, Qiyan; Chen, Lei; Lin, Shiye; Cao, Hui; Teng, Hui
• Is Part Of: Food chemistry. Volume 390(2022)
• Publication Date: 2022-10-01
• Subjects: DMY dihydromyricetin -- DMY-S dihydromyricetin self-microemulsion drug delivery system -- SMEDDS self-micro-emulsion drug delivery system -- TPGS d-α-tocopheryl polyethylene glycol 1000 succinate -- QS quillaja saponin -- TEM transmission electron microscope -- HFD high fat diet -- NAFLD non-alcoholic fatty liver disease -- BW body weight -- TBA total bile acid -- AST aspartate aminotransferase -- ALT alanine transaminase -- NEFA free fatty acid -- TC total cholesterol -- TG triglycerides -- HDL-C high-density lipoprotein -- LDL-C low-density lipoprotein cholesterol -- KEGG Kyoto Encyclopedia of Genes and Genomes -- ICR institute of cancer research -- PCA principal component analysis -- OPLS-DA orthogonal partial least squares discriminant analysis -- POS positive-ion mode -- NEG negative-ion mode -- VIP variable importance in the projection -- PAP adenosine 3′, 5′-bisphosphate; Dihydromyricetin -- Hyperlipidemia -- Metabonomics -- Self-microemulsion delivery system; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132954
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: TPGS-QS-SMEDDS is designed to enhance the absorptivity of DMY. The DMY-S can regulate the increase of body weight, serum and hepatic TG. The DMY-S can decrease the ratio of Firmicutes to Bacteroidetes . The DMY-S could inhibit sphingomyelin, CoA, nicotinate acid, and pyruvate expressions. Abstract: The present study aims to design a self-microemulsion delivery system (d-α-tocopheryl polyethylene glycol 1000 succinate - quillaja saponin) to enhance the absorptivity of dihydromyricetin (DMY-S), and to investigate its dietary intervention effect on high-fat-diet (HFD) fed mice. We find DMY-S can inhibit the increase of body weight and fat mass, preventing non-alcoholic fatty liver disease. Compared to the model group, the abundance of mice intestinal flora is mainly changed in certain bacterial genera of Firmicutes and Bacteroides, including norank_f_Muribaculaceae and Blautia. The result of metabolism analysis indicated that the expression levels of cincassiol B, creatine, pantothenic acid and aminobutyric acid in the liver tissues of mice treated with DMY-S showed a down-regulation. The DMY-S prevented hyperlipidemia in HFD mice mainly by affecting different pathways including glycerophospholipid metabolism, sphingolipid metabolism and pantothenate and CoA biosynthesis.