Quantitative assessment of phosphate food additive in frozen and chilled chicken using spectrophotometric approach combined with graphitic digestion. (30th September 2022)

• Record Type: Journal Article
• Authors: Hassan, Hassan M.; Alsohaimi, Ibrahim Hotan; Khan, Mohammad Rizwan; Alfalah, Abdulhamed Yousif Ahmed; Alruwaili, Musharraf Almunaffi Haddur; Alam, Pravej; Ahmad, Naushad; Ouladsmane, Mohamed
• Is Part Of: Food chemistry. Volume 389(2022)
• Publication Date: 2022-09-30
• Subjects: Chicken -- Phosphate -- Nitric acid -- Sulfuric acid -- Graphitic digestion -- Colorimetric -- Spectrophotometer; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.133050
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Spectrophotometry/graphitic digestion for phosphate analysis in iced up chicken. HNO3 (conc.) & H2 SO4 (conc.), and HNO3 (5 M) & H2 SO4 (conc.) were best reagents. Recovery rates were obtained up to 100%. System offered excellent R 2 > 0.999 with LOD, 0.032 mg/L and LOQ, 0.10 mg/L values. Frozen and chilled chicken produced phosphate 3.38–5.90 g/kg and 3.96–26.94 g/kg. Abstract: A new method based on spectrophotometry combined with graphitic digestion was developed for quantitative assessment of phosphate in frozen and chilled chicken meat. Digestion reagents comprising HNO3 (conc.) & H2 SO4 (conc.) and HNO3 (5 M) & H2 SO4 (conc.) were found to have optimal composition, affording similar recovery values of 100% and 99%, respectively, with excellent linearity ( R 2 > 0.999) and good limit of detection (LOD = 0.032 mg/L) and limit of quantification (LOQ = 0.10 mg/L), whereas other reagents offered lower recoveries (0–1.73%). Phosphate was found in concentrations of 3.38–5.90 g/kg and 3.96–26.94 g/kg in frozen and chilled chicken, respectively. Chilled chicken contained higher amounts of phosphate (>20 g/kg) than recommended by the European Commission (EC, 5 g/kg), either alone or in a mixture of processed meat products. This method is simple, cost-effective, and can be used as an alternative for analyzing phosphate in various samples comprising a similar matrix.