Effects of Enterococcus faecalis UGRA10 and the enterocin AS-48 against the fish pathogen Lactococcus garvieae. Studies in vitro and in vivo. (February 2019)

• Record Type: Journal Article
• Authors: Baños, Alberto; Ariza, Juan José; Nuñez, Cristina; Gil-Martínez, Lidia; García-López, J. David; Martínez-Bueno, Manuel; Valdivia, Eva
• Is Part Of: Food microbiology. Volume 77(2019)
• Page Start: 69
• Page End: 77
• Publication Date: 2019-02
• Subjects: Enterocin AS-48 -- Enterococcus faecalis UGRA10 -- Lactococcus garvieae -- Trout; Food Microbiology -- Periodicals; Aliments -- Microbiologie -- Périodiques; Food -- Microbiology; Periodicals; Food -- Microbiology -- Periodicals; Food contamination -- Periodicals; 664.001579
• DOI: 10.1016/j.fm.2018.08.002
• Languages: English
• ISSNs: 0740-0020

Abstract

Abstract: The aim of this study was to evaluate the effects of Enterococcus faecalis UGRA10 and its enterocin AS-48 against the fish pathogen Lactococcus garvieae . The minimum bactericidal concentrations of AS-48 against L. garvieae CECT 5807, 5806, and 5274 were 15.62, 15.62, and 7.81 μg/ml respectively. In broth cultures, enterocin at 100, 50, and 25 μg/ml reduced 10 8 CFU/ml lactococci after 2, 5, and 10 h, respectively. In co-cultures of UGRA10/L . garvieae at a 1/10 CFU/ml ratio, lactococci were eliminated after 24 h. Studies on UGRA10 biosafety and AS-48 toxicity in R1 cells and in rainbow trout have shown a lack of adverse effects from both the strain and bacteriocin. Trout challenged with L. garvieae and UGRA10 administered in diet 30 days before infection had a cumulative survival rate of 50% compared with 0% for control fish. Trout inoculated with the pathogen and treated by regular dipping in AS-48 baths had a survival rate of 60% after 20 days compared with that of untreated fish (0%). These results indicate the protective effect of the UGRA10 strain and the bacteriocin AS-48 against L. garvieae and the potential of these natural products as alternatives to antibiotics for controlling diseases in aquaculture. Highlights: Effects of UGRA10 strain and bacterocin AS-48 on Lactococcus garvieae were tested. Neither Enterococcus faecalis UGRA10 or AS-48 showed adverse effect on trout. UGRA10 strain administered in diet protected trout against L. garvieae infection. Dipping in AS-48 baths protected trout against L. garvieae infection.