Beyond amylose content, selecting starch traits impacting in vitro α-amylase degradability in a wheat MAGIC population. (1st September 2022)

• Record Type: Journal Article
• Authors: Wang, Yuzi; Kansou, Kamal; Pritchard, Jenifer; Zwart, Alexander B.; Saulnier, Luc; Ral, Jean-Philippe
• Is Part Of: Carbohydrate polymers. Volume 291(2022)
• Publication Date: 2022-09-01
• Subjects: Wheat -- Starch -- Screening -- Degradation -- Kinetics -- Multifactorial analysis; DP degree of polymerization -- RDS rapidly digested starch -- SDS slowly digested starch -- RS resistant starch -- MAGIC multiparent advanced generation intercross -- WWS (C+) waxy wheat starch -- HAWS (C−) high amylose wheat starch -- PPA porcine pancreatic α-amylase -- STD size threshold discrimination -- GMD gaussian mixture distribution -- PCA principal component analysis -- HCPC hierarchical clustering on principal components -- HAC hierarchical agglomerative clustering -- A/B ratio the ratio of A-type to B-type granules -- S/L ratio the ratio of short to long branch chains of amylopectin -- RSE residual standard error; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119652
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: A major challenge faced when studying the "structure-degradability" interaction of native starch is deciphering the interdependency between different structural levels, especially when experimental conditions limit the number of samples. To tackle this challenge, 224 wheat starches from a 4-way multiparent advanced generation inter-cross population were screened for structural features and degradation profiles by porcine pancreatic α-amylase. A hierarchical clustering on principal components (HCPC) were used as multifactorial analysis to explore the data structure. The degradation procedure was proved to be robust and sensible enough to screen a large collection of starches. The HCPC highlighted the combined effects of granule size distribution (GSD), amylopectin chain length distribution (CLD), amylose content and endogenous α-amylase activity on degradation kinetics. Especially the GSD and amylopectin CLD showed high co-occurrences with specific hydrolysis profiles. These findings provide an innovative screening method and structural factors to be primarily considered for wheat starch selection in breeding programs. Graphical abstract: Unlabelled Image