Proteomics and metabolomics reveal the mechanism underlying differential antioxidant activity among the organs of two base plants of Shiliang tea (Chimonanthus salicifolius and Chimonanthus zhejiangensis). (15th August 2022)

• Record Type: Journal Article
• Authors: Yang, Bingxian; Li, Qi; Cheng, Kaisen; Fang, Jie; Mustafa, Ghazala; Pan, Junjie; Xing, Bingcong; Lv, Qundan; Zhang, Lin; Cheng, Kejun
• Is Part Of: Food chemistry. Volume 385(2022)
• Publication Date: 2022-08-15
• Subjects: AA antioxidant activity -- ABTS the 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid -- ANR anthocyanidin reductase -- AOC allene oxide cyclase -- AOS allene oxide synthase -- BAPNA Nα-benzoyl-dl-arginine-p-nitroanilide -- CAT catalase -- CHAPS 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate -- CHO carbohydrate -- CSB branch of C. salicifolius -- CSL leaf of C. salicifolius -- CZB branch of C. zhejiangensis -- CZL leaf of C. zhejiangensis -- DAPs differentially abundant proteins -- DTT dithiothreitol -- EGTA glycol-bis-(2-aminoethylether)-N, N, N′, N′-tetraacetic acid -- EDTA ethylene diamine tetraacetic acid -- GO Gene Ontology -- GSH-PX glutathione peroxidase -- 13-HPOT 13(S)-hydroxy linolenic acid -- JA jasmonic acid -- LOX lipoxygenase -- PA procyanidin -- PCA principal component analysis -- PI protease inhibitor -- PMSF phenylmethylsulfonyl fluoride -- POD peroxidase -- ROS reactive oxygen species -- SMDs secondary metabolism related DAPs -- SOD superoxide dismutase -- TEAB tetraethylammonium bromide -- TMT tandem mass tag; Chimonanthus salicifolius -- Chimonanthus zhejiangensis -- Organ -- Antioxidant activity -- Procyanidins; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132698
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: The antioxidant activity of branch is stronger than leaf in two species. The trypsin/protease inhibitor activity of branch is stronger than leaf. Flavonoids (phenylpropanoids)-related proteins were with higher abundance in branch. Isoprenoids-related proteins were with higher abundance in leaf. Procyanidins were more accumulated in branch than leaf in two species. Abstract: The leaves and branches of Chimonanthus salicifolius and Chimonanthus zhejiangensis are the base ingredients of Shiliang tea. In this study, proteomics and metabolomics were performed to understand the molecular mechanisms underlying antioxidant activity (AA) in the leaves and branches of the two species. Stress and redox related proteins are differentially expressed among organs. The abundance of isoprenoid pathway-related proteins is higher in leaves while the abundance of phenylpropanoid and flavonoid pathway-related proteins is higher in branches in both species. Metabolomics revealed the flavonoid composition and demonstrated that procyanidins are more abundant in branches. Superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and AA are stronger in branches than leaves. Overall, branches might contribute to redox homeostasis through SOD/GSH-PX and flavonoids. Furthermore, the high level of AA of branches might be largely due to their increased accumulation of procyanidins.