Characterization of CRISPR-Cas systems in Bifidobacterium breve. Issue 4 (22nd April 2022)

• Record Type: Journal Article
• Authors: Han, Xiao; Zhou, Xingya; Pei, Zhangming; Stanton, Catherine; Ross, R. Paul; Zhao, Jianxin; Zhang, Hao; Yang, Bo; Chen, Wei
• Is Part Of: Microbial genomics. Volume 8:Issue 4(2022)
• Publication Date: 2022-04-22
• Subjects: Bifidobacterium breve -- CRISPR-Cas system -- genotyping -- prophage; Microbial genomics -- Periodicals; 572.8629
• DOI: 10.1099/mgen.0.000812
• Languages: English
• ISSNs: 2057-5858

Abstract

Abstract : The clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) system is an important adaptive immune system for bacteria to resist foreign DNA infection, which has been widely used in genotyping and gene editing. To provide a theoretical basis for the application of the CRISPR-Cas system in Bifidobacterium breve, the occurrence and diversity of CRISPR-Cas systems were analysed in 150 B. breve strains. Specifically, 47 % (71/150) of B. breve genomes possessed the CRISPR-Cas system, and type I-C CRISPR-Cas system was the most widely distributed among those strains. The spacer sequences present in B. breve can be used as a genotyping marker. Additionally, the phage assembly-related proteins were important targets of the type I-C CRISPR-Cas system in B. breve, and the protospacer adjacent motif sequences were further characterized in B. breve type I-C system as 5′-TTC-3′. All these results might provide a molecular basis for the development of endogenous genome editing tools in B. breve .