Agaricus blazei Murill polysaccharides alleviate oxidative stress and inflammatory responses against liver and lung injury. (June 2022)

• Record Type: Journal Article
• Authors: Wang, Wenshuai; Liu, Min; Zhang, Mengyu; Sun, Wenxue; Zhang, Jianjun; Jia, Le
• Is Part Of: Food bioscience. Volume 47(2022)
• Publication Date: 2022-06
• Subjects: Agaricus blazei Murill polysaccharides -- Antioxidation -- Inflammation -- Liver and lung injury -- NF-κB signaling pathway; Agaricus blazei Murill polysaccharides ABMP -- Agaricus blazei Murill A. blazei Murill -- Alanine aminotransferase ALT -- Aspartate aminotransferase AST -- Catalase CAT -- Cyclooxygenase-2 COX-2 -- Fourier transformation infrared FT-IR -- Glutathione peroxidase GSH-Px -- Gel permeation chromatography GPC -- High performance liquid chromatography HPLC -- Inhibitor of NF-κBα IκBα -- Interleukin-1β IL-1β -- Interleukin-6 IL-6 -- Malondialdehyde MDA -- Multiple organ dysfunction syndrome MODS -- Nuclear magnetic resonance NMR -- Phospho-nuclear factor-kappaB p65 p–NF–κB p65 -- Prostaglandin E2 PGE-2 -- Reactive oxygen species ROS -- Scanning electron microscopy SEM -- Superoxide dismutase SOD -- Tumor necrosis factor-α TNF-α -- Zymosan ZY -- Zymosan-induced generalized inflammation ZIGI; Food -- Biotechnology -- Periodicals; Food -- Research -- Periodicals; Aliments -- Biotecnologia -- Revistes; Aliments -- Investigació -- Revistes; Food -- Biotechnology; Food -- Research; Revistes electròniques; Periodicals; 664.005
• DOI: 10.1016/j.fbio.2022.101645
• Languages: English
• ISSNs: 2212-4292

Abstract

Abstract: As a kind of well-known edible mushroom, the medicinal values of Agaricus blazei Murill also have been well acknowledged. There is no efficient treatment strategy for multiple organ dysfunction syndrome (MODS) so far, a crucial cause of clinical death. The present work was designed to evaluate the beneficially protective effects of Agaricus blazei Murill polysaccharides (ABMP) on liver and lung against zymosan (ZY)-induced MODS related damages. The MODS mice were successfully established by given one-time injection of zymosan solution (500 mg/kg) intraperitoneally. The in vivo results demonstrated that ABMP could improve the 10-day survival rate, ease abnormal physiological behavior and alleviate tissues oxidative stress in MODS mice. Besides, ABMP showed potential effects on attenuating the functional impairments of liver and lung. Meanwhile, ABMP effectively inhibited the activation of NF-κB signaling pathway and reduced the expressions of inflammatory cytokines, including TNF-α, IL-1β, IL-6, COX-2 and PGE-2. Additionally, the structural characterizations showed that the significant function of ABMP was possibly related with the β-pyranose configuration with a triple-helical structure composing of glucose mainly with the proper molecular weights of 12.26 kDa. These findings demonstrated that ABMP exhibited potential protections against liver and lung damages in the ZY-induced MODS mice, providing more theoretical basis and reference significance for the exploration of biological activity of A. blazei Murill. Highlights: ABMP exhibited protective effects against systemic toxicity of zymosan. ABMP supplementation could relieve oxidative stress and repair histopathological changes of liver and lung. ABMP ameliorated inflammatory response by blocking NF-κB pathway and reducing the expression of inflammatory cytokines. ABMP could be used as natural functional materials for the prevention and treatment of MODS-related organ injuries.