Egg white lysozyme purification by a stirred cell contactor equipped with a weak ion-exchange nanofiber membrane: Process development and scale-up. (15th February 2021)

• Record Type: Journal Article
• Authors: Lee, Sze Ying; Liu, Bing-Lan; Wu, Jun-Yi; Chang, Yu-Kaung
• Is Part Of: Food chemistry. Volume 338(2021)
• Publication Date: 2021-02-15
• Subjects: Electrospinning -- Ion-exchange nanofiber membrane -- Lysozyme -- Purification -- Scale-up; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2020.128144
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A polyacrylonitrile nanofiber-based weak ion-exchange membrane was synthesized. The amounts of acidic functional groups on the ion-exchange membrane were optimized. A lysozyme recovery yield of 98% and a purification factor of 63 were achieved. Stirred cell contactor is a scalable membrane reactor for purification of lysozyme. Abstract: A weak ion-exchange membrane (P-COOH) was synthesized by alkaline hydrolysis of a polyacrylonitrile nanofiber membrane prepared by electrospinning process. The P-COOH membrane was characterized for its physical properties and its application for purification of lysozyme from chicken egg white was investigated. The lysozyme adsorption efficiency of the P-COOH membrane operating in a stirred cell contactor (Millipore, Model 8010) was evaluated. The effects of key parameters such as the feed concentration, the rotating speed, the flow rate of feed and the operating pressure were studied. The results showed successful purification of lysozyme with a high recovery yield of 98% and a purification factor of 63 in a single step. The purification strategy was scaled-up to the higher feedstock loading volume of 32.7 and 70 mL using stirred cell contactors of Model 8050 and 8200, respectively. The scale-up processes achieved similar purification results, proving linear scalability of the purification technique adopted.