Highly-hydrophobic nanofiber mat for efficient colonic delivery of lactoferrin: Preparation, characterization and release mechanism. (June 2022)

• Record Type: Journal Article
• Authors: Wei, Yun-Shan; Feng, Kun; Li, Shu-Fang; Hu, Teng-Gen; Zong, Min-Hua; Wu, Hong
• Is Part Of: Innovative food science & emerging technologies. Volume 78(2022)
• Publication Date: 2022-06
• Subjects: Highly-hydrophobic -- Lactoferrin -- Eudragit S100 -- Water-in-oil emulsion -- Coaxial electrospinning -- Colonic delivery; Lactoferrin Lf -- Eudragit S100 ES100 -- Acetic acid AA -- Gastrointestinal tract GIT -- Scanning electron microscopy SEM -- Contact angle CA -- Fourier-transform infrared spectroscopy FTIR -- Circular dichroism spectrum CD -- Sodium dodecyl sulfate-polyacrylamide gel electrophoresis SDS-PAGE -- de Man-Rogosa-Sharpe MRS -- Simulated gastric fluid SGF -- Simulated intestinal fluid SIF -- Simulated colonic fermentation fluid SCF -- Normal saline NS -- Dispersion of metabolites of gut microbiota CFM -- Fermentation system deprived of carbon source NCF -- Short-chain fatty acid SCFAs -- Cell Counting Kit-8 CCK-8; Food -- Biotechnology -- Periodicals; Food industry and trade -- Technological innovations -- Periodicals; Aliments -- Biotechnologie -- Périodiques; Food -- Biotechnology; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.ifset.2022.103015
• Languages: English
• ISSNs: 1466-8564

Abstract

Abstract: Lactoferrin (Lf) is a bioactive protein with varied biological effects. To improve its anti-digestive stability in oral administration, a novel nanocarrier with high hydrophobicity for colonic delivery of Lf was creatively developed by modified coaxial electrospinning. First, a suitable biocompatible solvent, acetic acid, was screened as the mono-solvent for ES100 electrospinning, creating highly-hydrophobic ES100 nanofiber mat (contact angle = 133.8 o ). Then, Lf-loaded W/O emulsion was prepared as the core fluid to ensure the successful coaxial electrospinning and generate Lf encapsulated core/shell nanofiber mat (ES@Lf). Lf was demonstrated maintaining structural integrity and anti-colon cancer activity during encapsulation and oral delivery. In vitro assay indicated 92.3% Lf was sustainably released in colon, and its release followed a complex mechanism in which the erosion was dominant. Instead of pH-dependent erosion, the synergistic action through gut microbiota adhesion and their metabolites, especially short-chain fatty acids, was illustrated for disintegration of ES@Lf nanofiber for the first time. Highlights: Acetic acid was screened firstly as mono-solvent for Eudragit S100 electrospinning. pH-dependent Eudragit S100 was processed into a highly-hydrophobic nanofiber mat. Lf in W/O emulsion was embedded into above fiber by modified coaxial electrospinning. 92.3% embedded Lf achieved colonic sustained release mediated by fiber mat erosion. Fiber mat was erode by synergism of colonic flora adhesion and their metabolites.