Detoxification of Ochratoxin A by pulsed light in grape juice and evaluation of its degradation products and safety. (June 2022)

• Record Type: Journal Article
• Authors: Wang, Leran; Liu, Xiaoshuang; Cai, Rui; Ge, Qian; Zhao, Zidan; Yue, Tianli; Yuan, Yahong; Gao, Zhenpeng; Wang, Zhouli
• Is Part Of: Innovative food science & emerging technologies. Volume 78(2022)
• Publication Date: 2022-06
• Subjects: Pulsed light -- Ochratoxin A -- Grape juice -- Juice quality -- Safety evaluation; Food -- Biotechnology -- Periodicals; Food industry and trade -- Technological innovations -- Periodicals; Aliments -- Biotechnologie -- Périodiques; Food -- Biotechnology; Periodicals; Electronic journals; 664.005
• DOI: 10.1016/j.ifset.2022.103024
• Languages: English
• ISSNs: 1466-8564

Abstract

Abstract: Pulsed light (PL) was used to remove Ochratoxin A (OTA) from clear grape juice in this study. Firstly, the factors of OTA degraded by PL were explored, and the OTA degradation rate was 95.29% after optimization by response surface methodology. Dose-response curves were most suitable for kinetic fitting of OTA degradation by PL treatment ( R 2 = 0.9937). Besides, results showed that pH value, soluble solids content and total organic acid content did not change significantly, little changes in color difference (ΔE < 3), while the content of monomer phenol decreased (12.81%–30.95%) with increased PL application time. The contents of alcohols, esters and aldehydes significantly decreased in aroma components, while the contents of ketones and acids significantly increased, alkanes were not affected. Results of animal experiments showed that PL treatment greatly alleviated the toxic effects of high dose OTA on mice. LC-MS/MS also showed that OTA produced non-toxic OTα and phenylalanine when treated with PL. Highlights: Pulsed light (PL) degraded 95.29% of Ochratoxin A (OTA) in grape juice after optimization by response surface methodology. Dose-response curves were most suitable for kinetic fitting of OTA degradation by PL treatment. PL treatment alleviated the toxic effects of high dose OTA on mice. OTA was treated with PL to produce less-toxic OTα and phenylalanine.