Aqueous two-phase extraction of polysaccharides from Selaginella doederleinii and their bioactivity study. (July 2022)

• Record Type: Journal Article
• Authors: Li, Gang; Ma, Xiankui; Jiang, Yongmei; Li, Weiwei; Wang, Yongling; Liu, Li; Sun, Chengxin; Xiao, Shiji; Lan, Jie; Kuang, Juxiang; Wang, Gang
• Is Part Of: Process biochemistry. Volume 118(2022)
• Page Start: 274
• Page End: 282
• Publication Date: 2022-07
• Subjects: Selaginella doederleinii -- Polysaccharides -- RSM -- Antioxidation -- Antitumor; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.04.024
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: In this study, the ethanol/salt aqueous two-phase system was firstly used to extract Selaginella doederleinii polysaccharides (SDP). Through single factor and response surface analysis, the best extraction conditions were as follows: extraction time of 40 min, ultrasonic power of 280 W, ethanol concentration of 35%, inorganic salt concentration of 0.22 g/mL, and liquid/solid ratio of 25 mL/g with optimal predicted yield of 9.85%. The preliminary structure of SDP was analyzed by HPLC, SEM, Congo red experiment, FT-IR NMR, SEM, thermogravimetric analysis and XRD. In vitro antioxidant experiments indicated that SDP exhibition a potential antioxidant activity and the scavenging rate of SDP (120 μg/mL) against DPPH +·, ABTS +· and ferrous ions were 39.35%, 43.25% and 48.63%, respectively. Also, SDP had certain antitumor activity and the IC50 of SDP on A549 cells and HCT-116 cells were 1528 μg/mL and 2341 μg/mL, respectively. Graphical Abstract: ga1 Highlights: S. doederleinii polysaccharide (SDP) was extracted by aqueous two-phase system. The optimum extraction process of SDP was gained. The structure of SDP was characterization. SDP has excellent antioxidant and anti-tumor activity.