Liquorilactobacillus satsumensis from water kefir yields α-glucan polysaccharides with prebiotic and synbiotic qualities. (15th August 2022)

• Record Type: Journal Article
• Authors: Tan, Li Ling; Ngiam, Jia Jun; Sim, Edric Shao Zhe; Conway, Patricia Lynne; Loo, Say Chye Joachim
• Is Part Of: Carbohydrate polymers. Volume 290(2022)
• Publication Date: 2022-08-15
• Subjects: ACN acetonitrile -- ANA anaerobic -- ASV amplicon sequence variant -- CAGR compound annual growth rate -- CF-MRS carbon-free De Man-Rogosa-Sharpe -- DI deionized -- DNA deoxyribonucleic acid -- DP degree of polymerization -- DSC differential scanning calorimetry -- EPS exopolysaccharide -- FOS fructo-oligosaccharides -- GC–MS gas chromatography mass spectrometry -- GIT gastrointestinal tract -- GOS galacto-oligosaccharides -- GPC gel permeation chromatography -- GRAS generally regarded as safe -- HP hydrolyzed polysaccharide -- HPLC-RID high performance liquid chromatography with refractive index detector -- LGG Lacticaseibacillus rhamnosus GG -- MRS De Man-Rogosa-Sharpe -- Mw molecular weight -- NB Nutrient Broth -- OX aerobic -- PBS phosphate buffered saline -- PCA principal coordinate analysis -- PDI polydispersity index -- rRNA ribosomal ribonucleic acid -- SCFA short chain fatty acid -- SGF simulated gastric fluid -- SIF simulated intestinal fluid -- Suc-MRS sucrose-supplemented De Man-Rogosa-Sharpe -- TCA trichloroacetic acid -- TFA trifluoroacetic acid -- TGA thermogravimetric analysis -- TMSP 3-(trimethylsilyl) propionic-2, 2, 3, 3-d4 acid sodium salt -- TS Tryptic Soy -- WC Wilkins-Chalgren Anaerobe -- 1H NMR proton nuclear magnetic resonance; Exopolysaccharide -- Dextran -- Hydrolysis -- bacteria-derived -- Synergistic -- Branching -- Lactobacilli -- Microbiome; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119515
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: Exopolysaccharides from water kefir grains are a potential source of novel, food-safe and functional materials. Herein, prebiotic properties of polysaccharides produced by water kefir-derived Liquorilactobacillus satsumensis bacteria were explored. Strains were cultured in sucrose-supplemented media for exopolysaccharides production, and partial hydrolysis was performed to yield shorter chain polysaccharides. Structural characterization revealed that hydrolyzed polysaccharides were branched glucans comprising α-1, 6 bonds and α-1, 3/α-1, 4 branching, with molecular weight of ~10 kDa. Hydrolyzed polysaccharides demonstrated selective utilization by probiotics, but not by pathogens, and were non-digestible by human digestive enzymes. Particularly, hydrolyzed polysaccharides were fermentable by kefir-derived probiotics, and these were combined in a novel kefir synbiotic formulation. Using large bowel simulated conditions, it was demonstrated that hydrolyzed polysaccharides and kefir synbiotics promoted Bacteroidetes abundance, and increased acetate, propionate, and butyrate concentrations. Overall, hydrolyzed glucans from Liquorilactobacillus satsumensis have prebiotic properties with enhanced benefits in a synbiotic when combined with kefir probiotics. Graphical abstract: Unlabelled Image