The inhibitory effect of curcumin loaded poly (vinyl caprolactam) nanohydrogel on insulin fibrillation. (June 2022)

• Record Type: Journal Article
• Authors: Bahmani, Marzieh; Akbarian, Mohsen; Tayebi, Lobat; Farjadian, Fatemeh
• Is Part Of: Process biochemistry. Volume 117(2022)
• Page Start: 209
• Page End: 218
• Publication Date: 2022-06
• Subjects: Insulin -- Fibrillation -- Curcumin -- Poly (vinylcaprolactam) -- PVCL -- Nano-hydrogel -- RAFT -- PEGDA; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2022.04.009
• Languages: English
• ISSNs: 1359-5113

Abstract

Abstract: Amyloidosis refers to a group of diseases caused by the deposition of abnormal proteins in tissues. Herein, curcumin was loaded in a nanohydrogel made of poly (vinylcaprolactam) to improve its solubility and was employed to exert an inhibitory effect on insulin fibrillation, as a protein model. Poly (vinyl caprolactam), cross-linked with polyethylene glycol diacrylate, was synthesized by the reversible addition-fragmentation chain transfer method. The release profile of curcumin exhibited a first-order kinetic model, signifying that the release of curcumin was mainly dominated by diffusion processes. The study of curcumin release showed that 78% of the compound was released within 72 h. The results also revealed a significant decline in insulin fibrillation in the presence of curcumin-loaded poly (vinyl caprolactam). These observations confirmed that increasing the ratio of curcumin-loaded poly (vinyl caprolactam) to insulin concentration would increase the hydrogel's inhibitory effect (P-value < 0.05). Furthermore, transmission electron and fluorescence microscopies and Fourier-transform infrared spectroscopy made it possible to study the size and interaction of fibrils. Based on the results, this nanohydrogel combination could protect the structure of insulin and had a deterrent effect on fibril formation. Graphical Abstract: ga1 Highlights: A nanohydrogel system based on PVCL was prepared and loaded with curcumin (Cur@PVCL). Cur@PVCL showed to have a first-order kinetic release profile of curcumin within 72 h. The mixture of Cur@PVCL with insulin showed to inhibit insulin fibrillation. Assessment showed that Cur@PVCl could inhibit the conformational changes of insulin.