Single-atom Ce-N-C nanozyme bioactive paper with a 3D-printed platform for rapid detection of organophosphorus and carbamate pesticide residues. (1st September 2022)

• Record Type: Journal Article
• Authors: Song, Guangchun; Zhang, Junjie; Huang, Huixian; Wang, Xin; He, Xiaoyun; Luo, Yunbo; Li, Jin-cheng; Huang, Kunlun; Cheng, Nan
• Is Part Of: Food chemistry. Volume 387(2022)
• Publication Date: 2022-09-01
• Subjects: Single-atom Ce-N-C nanozyme -- Peroxidase -- Bioactive paper -- 3D-printed platform -- Pesticide residues; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132896
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A novel SACe-N-C nanozyme with high POD-like activity was used to construct bioactive paper. The bioactive paper offered a portable approach to detect pesticide residues in fruits and vegetables within 30 min. An on-site portable testing device was proposed by integrating SACe-N-C bioactive paper into 3D printed platform. The proposed assay for omethoate, methamidophos, carbofuran, and carbosulfan showed an acceptable limit of detection. Abstract: Rapid detection of pesticide residues based on enzyme mimics has recently attracted much interest. However, most nanozymes have low activity. Herein, a "single-atom Ce-N-C nanozyme" (SACe-N-C nanozyme) was rationally devised and verified to mimic peroxidase (POD-like) with superior activity. Based on its high POD-like activities and cascaded catalytic reactions with acetylcholinesterase (AChE), we constructed a bioactive paper for the detection of pesticide residues, which offered a portable approach to monitor fruits and vegetables within 30 min. More importantly, a 3D printed platform was integrated on the basis of SACe-N-C bioactive paper to achieve on-site portable testing of omethoate, methamidophos, carbofuran, and carbosulfan, showing limits of detection (LODs) of 55.83, 71.51, 81.81, and 74.98 ng/mL, respectively. The recovery rates were 84.09–104.68%. This study provided new insight into the design of novel single-atom nanozymes for cascaded catalytic detection and other rapid detection applications with high efficiency and low cost.