A solid-phase capture probe based on upconvertion nanoparticles and inner filter effect for the determination of ampicillin in food. (30th August 2022)

• Record Type: Journal Article
• Authors: Zhang, Yunlian; Hassan, Md Mehedi; Rong, Yawen; Liu, Rui; Li, Huanhuan; Ouyang, Qin; Chen, Quansheng
• Is Part Of: Food chemistry. Volume 386(2022)
• Publication Date: 2022-08-30
• Subjects: Upconversion fluorescence -- Ampicillin -- Solid-phase capture probe -- PtNPs -- Inner filter effect; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132739
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: White-light UCNPs were synthesized with good properties. An IFE sensing system for AMP detection was fabricated. An LOD of 0.32 ng·mL −1 was achieved. Abstract: Ampicillin (AMP) is commonly used to treat diseases caused by bacterial infections as a veterinary drug. However, the abuse of AMP can lead to residues in food and ultimately cause harm to humans. Thus, it is significant to construct a reliable system for AMP detection. Here, we developed an inner filter effect system based on a solid-phase capture probe and the catalysis of platinum nanoparticles (PtNPs) for AMP determination in food. In the presence of AMP, PDMS captured AMP then combined with aptamer-functionalized PtNPs, which catalyzed the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine, resulting in upconversion fluorescence quenching. The results showed the fluorescence intensity of upconversion nanoparticles was related to AMP concentration (0.5–100 ng·mL −1 ) with an LOD of 0.32 ng·mL −1, which made quantification of AMP possible. The method also achieved a satisfactory recovery rate (96.89–112.92%) and can be used for AMP detection in food samples with selectivity and sensitivity.