Impact of molar absorbance on anthocyanin content of the foods. (30th August 2022)

• Record Type: Journal Article
• Authors: Chandra Singh, Mamatha; Price, William E.; Kelso, Celine; Charlton, Karen; Probst, Yasmine
• Is Part Of: Food chemistry. Volume 386(2022)
• Publication Date: 2022-08-30
• Subjects: Delphinidin-3-glucoside chloride (PubChem CID: 165558) -- Cyanidin-3-glucoside chloride (PubChem CID: 197081) -- Pelargonidin-3-glucoside chloride (PubChem CID: 443648) -- Petunidin-3-glucoside chloride (PubChem CID: 14311148) -- Peonidin-3-glucoside chloride (PubChem CID: 44256838) -- Malvidin-3-glucoside chloride (PubChem CID: 11249520) -- TBHQ (PubChem CID: 16043) -- Formic acid (PubChem CID: 284) -- Methanol (PubChem CID: 424390) -- Potassium chloride (PubChem CID: 4873) -- Sodium acetate (PubChem CID: 517045) -- Hydrochloric acid (PubChem CID:313); Extinction coefficient -- LC-MS -- UV–Vis -- Anthocyanins -- Blueberries -- Validation; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132855
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Significant differences in anthocyanin estimations using different reporting methods. Inter-laboratory validation allows better determination of extinction coefficient (Ɛ). pH differential method is most widely used despite variation in reported Ɛ values. Reporting total anthocyanin based on cya-3-glu is a constraint of the AOAC method. Abstract: Anthocyanins are present in bright colored fruit and vegetables with growing evidence for their health benefits. Several methods exist in the literature to measure the total monomeric anthocyanin content in foods. Although the simplest method uses UV–Vis spectrophotometry, it requires the use of anthocyanin molar absorption coefficients (Ɛ). While commonly reported for some compounds, these values vary substantially between studies. This study collated and compared existing Ɛ values for a range of anthocyanin-3-glucosides, measured new Ɛ values for these compounds and underwent an inter-laboratory validation of spectrometry methods. The Ɛ values used for the determination of anthocyanin content in Australian blueberries, were shown to greatly affect the estimated total anthocyanin. Significant differences in the Ɛ values were observed when measured at 520 nm, or their absorbance maximum and substantial difference in the estimated total anthocyanins were observed when expressed as equivalent of cya-3-glu or mal-3-glu.