Release characteristic and mechanism of bound polyphenols from insoluble dietary fiber of navel orange peel via mixed solid-state fermentation with Trichoderma reesei and Aspergillus niger. (1st May 2022)

• Record Type: Journal Article
• Authors: Xue, Puyou; Liao, Wang; Chen, Yi; Xie, Jianhua; Chang, Xinxin; Peng, Guanyi; Huang, Qing; Wang, Yudan; Sun, Nan; Yu, Qiang
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 161(2022)
• Publication Date: 2022-05-01
• Subjects: Bound polyphenols -- Release mechanism -- Navel orange peel -- Insoluble dietary fiber -- Mixed solid-state fermentation; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.113387
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Dietary fiber (DF) is a carrier of abundant bound polyphenols (BP). The present work was to investigate the release characteristic and mechanism of BP from the insoluble dietary fiber of navel orange peel (NOP-IDF) by mixed solid-state fermentation (SSF) with Trichoderma reesei and Aspergillus niger . The optimized fermentation conditions were set as follows: inoculation ratio of T. reesei to A. niger 3:1, delayed inoculation time 28 h and initial pH 6.5. The compositions of BP were identified and quantified by UPLC-ESI-QTOF-MS/MS and HPLC-ESI-QQQ-MS/MS, then the biotransformation pathways were partially speculated. Moreover, cellulase, xylanase and β -glucosidase activities were strongly correlated with the BP content. The SEM showed the cell wall and the crystalline structure of the NOP-IDF was effectively decomposed. Furthermore, mixed SSF significantly enhanced in vitro antioxidant activities of BP. In summary, our study demonstrated that mixed SSF was a feasible method for the BP release. Highlights: Mixed SSF with T. reesei and A. niger effectively facilitated the BP release. Forty-four BP were identified and quantified from the NOP-IDF. The biotransformation pathways of BP were partially speculated during mixed SSF. Cellulase, xylanase and β -glucosidase activities were correlated with the BP. SEM further suggested that mixed SSF might facilitate the BP release.