Protective Effects of Feruloyl Oligosaccharides from Fermented Wheat Bran against Oxidative Stress in IPEC-J2 Cells In Vitro and in a Zebrafish Model In Vivo. (14th April 2022)

• Record Type: Journal Article
• Authors: Zhang, Jia; Chen, Qiuyan; Wang, Yuan; Wang, Ruxin; Wang, Ruifang; Hao, Xiran; Zheng, Yue; An, Xiaoping; Qi, Jingwei
• Other Names: He Shudong Academic Editor.
• Is Part Of: Journal of food quality. Volume 2022(2022)
• Publication Date: 2022-04-14
• Subjects: Food industry and trade -- Quality control -- Periodicals; Food industry and trade -- Standards -- Periodicals; Food -- Periodicals; 664.07
• DOI: 10.1155/2022/8001728
• Languages: English
• ISSNs: 0146-9428

Abstract

Abstract : This study aims to evaluate the protective effects of feruloyl oligosaccharides from fermented wheat bran (FOs-FWB) against oxidative stress in IPEC-J2 cells in vitro and in a zebrafish model in vivo. Results showed that FOs-FWB effectively reduces reactive oxygen species (ROS) and malondialdehyde (MDA) content in lipopolysaccharide (LPS)-stimulated IPEC-J2 cells while elevating glutathione (GSH) content and superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities. The real-time PCR data illustrated that FOs-FWB upregulated the mRNA expression levels of glutamate-cysteine ligase catalytic subunit (GCLC), glutamate-cysteine ligase modifier subunit (GCLM), NAD (P) H: quinone oxidoreductase-1 (NQO-1), and heme oxygenase-1 (HO-1). In the model of AAPH-stimulated zebrafish embryos, we observed that FOs-FWB suppressed cell death, ROS generation, and lipid peroxidation, along with improvements in SOD, CAT, and GSH-Px activities. Therefore, FOs-FWB exerted protective effects against oxidative stress in IPEC-J2 cells and zebrafish.