A novel strategy for isolation and purification of fucoxanthinol and fucoxanthin from the diatom Nitzschia laevis. (30th March 2019)

• Record Type: Journal Article
• Authors: Sun, Peipei; Wong, Chi-Chun; Li, Yuelian; He, Yongjin; Mao, Xuemei; Wu, Tao; Ren, Yuanyuan; Chen, Feng
• Is Part Of: Food chemistry. Volume 277(2019)
• Page Start: 566
• Page End: 572
• Publication Date: 2019-03-30
• Subjects: Nitzschia laevis -- Fucoxanthinol -- Fucoxanthin -- Solid-phase extraction -- Thin-layer chromatography; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.10.133
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Nitzschia laevis was a promising resource to develop fucoxanthinol and fucoxanthin production. A simple two-step method was mediated with SPE and TLC techniques. This exploited approach could effectively purified fucoxanthinol and fucoxanthin. The purities of the two purified compounds were over 95%. Abstract: In this study, the microalga Nitzschia laevis ( N. laevis ) can accumulate a marine carotenoid fucoxanthinol. In particular, fucoxanthinol was firstly isolated from microalgae, accompanied by its derivative fucoxanthin. The identification and quantification of fucoxanthinol and fucoxanthin were determined by ultra-performance liquid chromatography coupled to photodiode array detector-quadrupole/travelling-wave ion mobility mass spectrometry/time-of-flight mass spectrometry (UPLC-PDA-TWIMS-QTOF-MS). Furthermore, a cost-effective approach mediated with solid-phase extraction (SPE) and thin-layer chromatography (TLC) technique was used to isolate and purify fucoxanthinol and fucoxanthin from the extracts of N. laevis . This two-step method can obtain 98% fucoxanthinol and 95% fucoxanthin, with the recovery efficiencies of around 85% for fucoxanthinol and 70% for fucoxanthin, respectively. Moreover, 1 H and 13 C nuclear magnetic resonance (NMR) techniques were adopted to record the purified compounds for supporting the above results. In all, the developed method has a promising potential to purify fucoxanthinol and fucoxanthin of microalgae for food and pharmaceutical applications.