Simultaneous determination of praziquantel and its main metabolites in the tissues of black goats and their residue depletion. Issue 4 (3rd April 2022)

• Record Type: Journal Article
• Authors: Ding, Tong-Yan; Shu, Xiao-Gui; Xiong, Ren-Ping; Qiu, Jing-Li; Li, Lu; He, Li-Min
• Is Part Of: Food additives & contaminants. Volume 39:Issue 4(2022)
• Page Start: 666
• Page End: 677
• Publication Date: 2022-04-03
• Subjects: Black goat -- praziquantel -- metabolites -- LC-MS/MS -- residue depletion; Food additives -- Periodicals; Food contamination -- Periodicals; 664.06
• DOI: 10.1080/19440049.2022.2032380
• Languages: English
• ISSNs: 1944-0049

Abstract

Abstract: Praziquantel (PZQ) is a pyrazino-isoquinoline compound with broad spectrum of activity against parasitic trematodes and cestodes, and a key veterinary drug in the parasitic disease control field. However, PZQ residues caused by non-conforming or excessive use in food-producing animals may pose a serious threat to human health. Herein, a simple, sensitive and reproducible LC-MS/MS method was developed for the simultaneous determination of praziquantel and trans - and cis -4-hydroxypraziquantel in black goat tissues to guide the reasonable use of PZQ. The mean recoveries for three target analytes were 71.2 ∼ 117.6%, and the limits of quantification were 1.0 μg/kg. Twenty-five healthy black goats were administered a single dose of praziquantel tablets at a dose of 35 mg/kg of body weight for residue elimination study, The results revealed that praziquantel and 4-hydroxypraziquantel were rapidly depleted in goat tissues and the elimination half-lives did not exceed 1 day in all tissues except for muscle and lung. It provides guidance for the establishment of maximum residue limit of praziquantel in goat.