Dietary Blueberry Ameliorates Vascular Complications in Diabetic Mice Possibly through NOX4 and Modulates Composition and Functional Diversity of Gut Microbes. Issue 8 (25th February 2022)

• Record Type: Journal Article
• Authors: Petersen, Chrissa; Bharat, Divya; Wankhade, Umesh D.; Kim, Ji‐Seok; Cutler, Brett Ronald; Denetso, Christopher; Gholami, Samira; Nelson, Samantha; Bigley, Jessica; Johnson, Aspen; Chintapalli, Sree V.; Piccolo, Brian D.; Satheesh Babu, Adhini Kuppuswamy; Paz, Henry A.; Shankar, Kartik; Symons, J. David; Anandh Babu, Pon Velayutham
• Is Part Of: Molecular nutrition & food research. Volume 66:Issue 8(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-25
• Subjects: blueberry -- diabetes -- endothelial dysfunction -- endothelium -- gut microbiota -- metabolites -- vascular disease -- vascular inflammation; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.202100784
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: In diabetes, endothelial inflammation and dysfunction play a pivotal role in the development of vascular disease. This study investigates the effect of dietary blueberries on vascular complications and gut microbiome in diabetic mice. Methods and Results: Seven‐week‐old diabetic db/db mice consume a standard diet ( db/db ) or a diet supplemented with 3.8% freeze‐dried blueberry ( db/db +BB) for 10 weeks. Control db/+ mice are fed a standard diet ( db/+ ). Vascular inflammation is assessed by measuring monocyte binding to vasculature and inflammatory markers. Isometric tension procedures are used to assess mesenteric artery function. db/db mice exhibit enhanced vascular inflammation and reduced endothelial‐dependent vasorelaxation as compared to db/+ mice, but these are improved in db/db +BB mice. Blueberry supplementation reduces the expression of NOX4 and IκKβ in the aortic vessel and vascular endothelial cells (ECs) isolated from db/db +BB compared to db/db mice. The blueberry metabolites serum reduces glucose and palmitate induced endothelial inflammation in mouse aortic ECs. Further, blueberry supplementation increases commensal microbes and modulates the functional potential of gut microbes in diabetic mice. Conclusion: Dietary blueberry suppresses vascular inflammation, attenuates arterial endothelial dysfunction, and supports the growth of commensal microbes in diabetic mice. The endothelial‐specific vascular benefits of blueberries are mediated through NOX4 signaling. Abstract : Dietary supplementation of blueberry suppresses vascular inflammation, improves vascular dysfunction and increases the abundance of beneficial gut microbes in diabetic mice. The circulating metabolites of blueberry appears to exert benefits on the vascular endothelium. Our study provides strong proof of concept to consider blueberry supplementation as an adjunct therapy to lessen vascular complications in diabetes.