Comparative genomics of wild-type and laboratory-evolved biofilm-overproducing Deinococcus metallilatus strains. Issue 12 (4th December 2020)

• Record Type: Journal Article
• Authors: Park, Chulwoo; Shin, Bora; Kim, Wonjae; Cheong, Hoon; Park, Soyoon; Park, Woojun
• Is Part Of: Microbial genomics. Volume 6:Issue 12(2020)
• Publication Date: 2020-12-04
• Subjects: biofilm -- CpG island -- Deinococcus -- extracellular matrix -- genome deletion -- stress response; Microbial genomics -- Periodicals; 572.8629
• DOI: 10.1099/mgen.0.000464
• Languages: English
• ISSNs: 2057-5858

Abstract

Abstract : Deinococcus metallilatus MA1002 was exposed to ultraviolet radiation to generate mutants with enhanced biofilm production. Two strains (nos 5 and 6) were then selected based on their high biofilm formation, as well as their possession of higher concentrations of extracellular matrix components (eDNA, protein and saccharides) than the wild-type (WT). Genomic sequencing revealed the presence of large genome deletions in a secondary chromosome in the mutants. Expression analyses of the WT and mutant strains indicated the upregulation of genes associated with exopolysaccharide synthesis and stress response. The mutant strains showed high mortality in glucose-supplemented (TYG) medium; however, cell death and biofilm formation were not increased in mutant cells grown under acetate- or glyoxylate-added media, suggesting that metabolic toxicity during glucose metabolism induced a high rate of cell death but improved biofilm formation in mutant strains. In damaged cells, eDNAs contributed to the enhanced biofilm formation of D. metallilatus .