Myo‐Inositol in Fermented Sugar Matrix Improves Human Macrophage Function. Issue 8 (26th February 2022)

• Record Type: Journal Article
• Authors: Ghosh, Nandini; Das, Amitava; Biswas, Nirupam; Mahajan, Sanskruti P.; Madeshiya, Amit K.; Khanna, Savita; Sen, Chandan K.; Roy, Sashwati
• Is Part Of: Molecular nutrition & food research. Volume 66:Issue 8(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-02-26
• Subjects: dietary supplement -- macrophage -- myo‐inositol -- phagocytosis -- ROS; Food -- Biotechnology -- Periodicals; Food -- Microbiology -- Periodicals; Nutrition -- Periodicals; Food -- Toxicology -- Periodicals; Nutrition -- Periodicals; Food Microbiology -- Periodicals; Food Technology -- Periodicals; Molecular Biology -- Periodicals; 664.0705
• DOI: 10.1002/mnfr.202100852
• Languages: English
• ISSNs: 1613-4125

Abstract

Abstract : Scope: Reactive oxygen species production by innate immune cells plays a central role in host defense against invading pathogens at wound‐site. A weakened host‐defense results in persistent infection leading to wound chronicity. Fermented Papaya Preparation (FPP), a complex sugar matrix, bolsters respiratory burst activity and improves wound healing outcomes in chronic wound patients. The objective of the current study was to identify underlying molecular factor/s responsible for augmenting macrophage host defense mechanisms following FPP supplementation. Methods and Results: In depth LC‐MS/MS analysis of cells supplemented with FPP led to identification of myo ‐inositol as a key determinant of FPP activity towards improving macrophage function. Myo ‐inositol, in quantities that is present in FPP, significantly improved macrophage respiratory burst and phagocytosis via de novo synthesis pathway of ISYNA1. In addition, myo ‐inositol transporters, HMIT and SMIT1, played a significant role in such activity. Blocking these pathways using siRNA attenuated FPP‐induced improved macrophage host defense activities. FPP supplementation emerged as a novel approach to increase intracellular myo ‐inositol levels. Such supplementation also modified wound microenvironment in chronic wound patients to augment myo ‐inositol levels in wound fluid. Conclusion: These observations indicate that myo ‐inositol in FPP influences multiple aspects of macrophage function critical for host defense against invading pathogens. Abstract : Fermented Papaya Preparation (FPP) treatment increases macrophage intracellular myo ‐inositol. FPP and myo‐inositol bolsters human macrophage ROS production and phagocytosis. Myo ‐inositol transporters SMIT1 and HMIT are involved in FPP and myo‐inositol induced macrophage functions. Myo ‐inositol improves macrophage functions via ISYNA1 and RAC2‐mediated pathway.