An Occam's razor: Synthesis of osteoinductive nanocrystalline implant coatings on hierarchical superstructures formed by Mugil cephalus skin hydrolysate. (December 2021)

• Record Type: Journal Article
• Authors: Acharya, Prakruti P.; Bajaj, Mayur; Irle, Hemant; Banerjee, Pradipta
• Is Part Of: Process biochemistry. Volume 111:Part 2(2021)
• Page Start: 278
• Page End: 296
• Publication Date: 2021-12
• Subjects: Collagen hydrolysate -- Quasi-fibril -- Biomaterial -- Biomimetic composite -- Nano-hydroxyapatite -- Implant coatings -- Osteogenic differentiation; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2021.11.006
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: 59 % w/w collagen type I isolated from mullet skin and digested enzymatically. Collagen Hydrolysate (CH) peptides orients in PP-II and form mimic helices. CH mimic helices assemble to form a small and dynamic quasi-fibril at pH 7−8. CH quasi-fibrils forms nanometric hydroxyapatite (HA) with high crystallinity. Coatings made from CH/HA nanocrystals support enhanced osteoindiction. Abstract: This study was conducted to implement fish collagen hydrolysate as a substrate for synthesizing a nanophasic hydroxyapatite (HA) coating that would display superior osteoinductivity than HA derived from collagen (C/HA) and to comprehend the contribution of secondary structure on HA synthesis. Collagen type I was isolated from mullet skin and fragmented to obtain collagen hydrolysate (MCH). CD, FTIR, XRD, DLS and MS studies revealed that the MCH peptides oriented in polyproline-II conformation and coiled to form a mimic-helix with a packing distance of 0.885 nm. Multiple helices self-assembled into a quasi-fibrillar network with a diameter of 931 nm. The MCH was suspended in HA-nucleating solution to form MCH/HA nanocomposite crystals, which exhibited HA-specific planes in XRD with crystal orientation towards the quasi-fibrillar axis and a % crystallinity of 96.04. MCH/HA crystals were plate-shaped with an average size of 55.4 nm and a Ca/P ratio of 1.77 with moderate resilience (9.9 GPa) and high wettability. Consequently, osteoblasts cultured on MCH/HA coated-Ti surface proliferated faster and expressed 1.2–2 times higher levels of osteogenic differentiation markers when compared to C/HA-coated and uncoated surfaces. The study identified MCH/HA to be a superior coating material, by virtue of the unique quasi-fibrillar architecture of MCH.