Engineering of anticancer drugs entrapped polymeric nanoparticles for the treatment of colorectal cancer therapy. (December 2021)

• Record Type: Journal Article
• Authors: Meng, Fanqi; Yun, Zhennan; Yan, Guoqiang; Wang, Guangyi; Lin, Chao
• Is Part Of: Process biochemistry. Volume 111:Part 2(2021)
• Page Start: 36
• Page End: 42
• Publication Date: 2021-12
• Subjects: Combination therapy -- Polymeric nanoparticles -- Dual anticancer drugs -- Colorectal cancer -- Cell death -- Systemic toxicity; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2021.09.013
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Curcumin (CUR) and Methotrexate (MEX) encapsulated polymeric nanoparticles (CUR/MEX@NPs) were developped. CUR/MEX@PNPs confirmed by the various analytical methods. CUR/MEX@PNPs enhanced the in vitro proliferation against the colorectal cancer cells. Biochemical analysis results reveals that CUR/MEX@PNPs induce apoptosis. The apoptosis was confirmed by Annexin-V-FITC and PI for flow cytometry. Abstract: Traditional chemotherapeutic agents have been successfully used to treat cancers, although their many disadvantages include poor solubility, high dosage needs, severe side effects, low therapeutic indices, development of multiple drug resistance, and non-specific targeting of cancer cells. In this study, Methotrexate (MEX) and curcumin (CUR) were incorporated into the triblock copolymer poly(lactic-co-glycolic acid) (PLGA)–poly(ethylene glycol) (PEG)–PLGA to achieve synergism in the treatment of colorectal cancer. Nanoparticle-based combination treatments for colorectal cancer can be developed using this remarkably symmetric synthetic strategy. The particles were visualized by high-resolution transmission microscopy (HR-TEM), and their size and polydispersity were determined using dynamic light scattering. Without a blast of impact, MEX and CUR were released gently from the polymeric nanoparticle system. CUR/MEX@NPs also demonstrated dose-dependent cytotoxic effects on SW1417 and CL40 cell lines, with a higher apoptosis ratio than free MEX and CUR. According to the in vitro cellular uptake analysis, cancer cells absorbed the polymeric nanoparticles more readily. From the fluorescence experiment, nanomaterials promoted cell death in the SW1417 and CL40 cell lines. Additionally, in vivo systemic toxicity and in vitro findings support the biocompatibility of CUR/MEX@NPs. Furthermore, these nanomaterials cause apoptosis in cancer cells. From these findings, CUR/MEX@NPs exhibit the potential to be successfully utilized to treat colorectal cancer.