Antibacterial activity of ethyl acetate extract of endophytic fungus (Paraconiothyrium brasiliense) through targeting dihydropteroate synthase (DHPS). (December 2021)

• Record Type: Journal Article
• Authors: Sathiyaseelan, Anbazhagan; Saravanakumar, Kandasamy; Mariadoss, Arokia Vijaya Anand; Kim, Kristine M.; Wang, Myeong-Hyeon
• Is Part Of: Process biochemistry. Volume 111:Part 2(2021)
• Page Start: 27
• Page End: 35
• Publication Date: 2021-12
• Subjects: Paraconiothyrium brasiliense -- GC–MS -- Antioxidant -- Cytotoxicity -- Antibacterial -- DHPS; Biochemical engineering -- Periodicals; Biotechnology -- Periodicals; Biochemistry -- periodicals; Biotechnology -- periodicals; Chemical Engineering -- periodicals; Génie biochimique -- Périodiques; Biotechnologie -- Périodiques; Biochemical engineering; Biotechnology; Periodicals; 660.63
• DOI: 10.1016/j.procbio.2021.10.010
• Languages: English
• ISSNs: 1359-5113

Abstract

Graphical abstract: Highlights: Endophytic fungus Paraconiothyrium brasiliense isolated from the fruit of Capsicum annuum L. Bioactive volatile molecules in ethyl acetate extract of P. brasiliense determined by GC–MS. EAE of P. brasiliense showed considerable antioxidant, anticancer and antibacterial activities. 2, 5-pipeazonedione substantially interacts with the bacterial DHPS enzyme. Abstract: Endophytic fungi are innumerable sources of bioactive metabolites with various biomedical applications. Hence, this study was aimed to isolate and identify the endophytic fungus Paraconiothyrium brasiliense (PB) from the fruit of Capsicum annuum and evaluated their biochemical, antimicrobial, antioxidant, cell viability, and cytotoxicity behavior. Results showed that ethyl acetate extract (EAE) of PB contains flavonoid (31.53 ± 0.9 mg of QE/g of extract) and phenolic (2.59 ± 0.06 mg of GAE/g of extract). The PB-EAE showed significant antioxidant activity in terms of free radicals (DPPH, ABTS, and Fe 3+ ) scavenging. The antioxidant potential of PB-EAE reflects in normal cell viability and anticancer activity against prostate cancer (PC3) cells. The bacterial inhibitory activity of PB-EAE was equal to commercial antibiotic tetracycline hydrochloride (TCH). Further, GC–MS analysis confirmed the presence of volatile molecules such as o-cymene (CH3 C6 H4 CH(CH3 )2 ), dipentene (C10 H16 ), γ-terpinene (C10 H16 ), 4-carvomenthenol (C10 H18 O), heneicosane (CH3 (CH2 )19 CH3 ), and 2, 5-piperazinedione (C4 H6 N2 O2 ). Also, molecular docking analysis demonstrated the significant interactions between the compounds of PB-EAE with dihydropteroate synthase (Protein ID:5JQ9; DHPS). This study revealed that endophytic P. brasiliense could be a novel source for isolation of antimicrobials.