Phospholipase cocktail: A new degumming technique for crude soybean oil. (1st April 2022)

• Record Type: Journal Article
• Authors: dos Passos, Rafaela Menezes; da Silva, Rúbia Mariana; de Almeida Pontes, Paula Virginia; Morgano, Marcelo Antônio; Meirelles, Antônio J.A.; Stevens, Christian V.; Ferreira, Marcela Cravo; Sampaio, Klicia Araujo
• Is Part Of: Lebensmittel-Wissenschaft + Technologie =. Volume 159(2022)
• Publication Date: 2022-04-01
• Subjects: Phosphorus content -- Oil yield -- Phospholipid -- Enzymatic degumming -- Vegetable oil; Food industry and trade -- Periodicals; Food -- Composition -- Periodicals; Microbiology -- Periodicals; Nutrition -- Periodicals; 664.005
• DOI: 10.1016/j.lwt.2022.113197
• Languages: English
• ISSNs: 0023-6438

Abstract

Abstract: Enzymatic degumming (EDG) is an emerging alternative process for decreasing the phosphorus content, increasing the oil yield, and preserving the oil quality. Purifine® 3G is a cocktail of phospholipases composed of phospholipase A2 (PLA2 ), phospholipase C (PLC), and phosphatidylinositol phospholipase C (PI-PLC). In this study, Purifine® 3G was applied to crude soybean oil, and the optimum degumming conditions (enzyme concentration, temperature, and water dosage) were determined using a central composite rotatable design (CCRD). The contents of diacylglycerols (DAGs) and free fatty acids (FFAs) in the studied system considerably increased at temperatures below 64 °C and enzyme concentrations above 100 mg/kg, while the phosphorus content decreased with increasing water amount and enzyme concentration. In particular, EDG with 200 mg/kg of Purifine® 3G conducted for 120 min at a temperature of 60 °C and water concentration of 3% (w/w) lowered the residual phosphorus content to 8.9 mg/kg and increased the FFA and DAG concentrations by 0.17% and 0.72%, respectively. Meanwhile, EDG retained the tocopherol content in crude soybean oil, maintaining its quality. Hence, Purifine® 3G increases the neutral oil yield (FFA and DAG), decreases the phosphorus content, and preserves the oil quality, which make it a commercially viable degumming agent. Graphical abstract: Image 1 Highlights: Successful enzymatic degumming with a new Purifine® 3G cocktail is performed. A phospholipid conversion of 98.8% is achieved while increasing the neutral oil yield. The developed process decreases the phosphorus content by 98.5%. The use of Purifine® 3G cocktail retains a high amount of tocopherols.