Determination of trace antibiotics in water and milk via preconcentration and cleanup using activated carbons. (15th August 2022)

• Record Type: Journal Article
• Authors: Liu, Pengyun; Wu, Zhilin; Barge, Alessandro; Boffa, Luisa; Martina, Katia; Cravotto, Giancarlo
• Is Part Of: Food chemistry. Volume 385(2022)
• Publication Date: 2022-08-15
• Subjects: ABX antibiotics -- SMM sulfamonomethoxine sodium -- OTC oxytetracycline -- CEF ceftiofur hydrochloride -- MAR marbofloxacin -- MRLs maximum residue limits -- HPLC high pressure liquid chromatography -- SPE solid phase extraction -- RE recovery efficiency -- ACs activated carbons -- CPAC coconut powdered AC -- SBET Brunauer-Emmett-Teller surface area -- VMeso mesopore volume -- VMicro micropore volume -- PGAC peat granular AC -- WPAC wood powder AC -- MeCN acetonitrile -- TFA trifluoroacetic acid -- MeOH methanol -- EtOH ethanol -- NH4OH ammonia solution -- EDTA ethylenediaminetetraacetic acid disodium salt hydrate -- t-BuOH t-butanol -- n-PrOH n-propanol -- MW molecular weight -- MV molar volume -- LogKOW octanol-water partition coefficient -- SWater solubility of ABX in water -- SEtOH saturated mole fraction solubility of ABX in EtOH -- pKa dissociation constant -- NHA number of H-bond acceptors -- NHD number of H-bond donors -- Refs. references -- Bp boiling point -- ε dielectric constant -- EDA π–π electron donor-acceptor -- TC tetracycline -- LODs limit of detections -- LOQs limit of quantitations -- LRs linear ranges -- RSD relative standard deviation -- FA formic acid -- DCM methylene chloride -- MSPD magnetic solid phase dispersion -- MSPE magnetic solid phase extraction.; Antibiotic residues in milk -- Preconcentration -- Cleanup -- Activated carbon -- Solid phase extraction; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132695
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Simple preconcentration and cleanup of trace antibiotics in milk using activated carbon (AC)-based solid phase extraction for HPLC analysis. Highlights: Coconut powder activated carbon (CPAC, 10–15 mg) can be used as adsorbent for SPE. Trace antibiotics (ABX) in water and milk were effectively recovered by CPAC-SPE. Co-solvent 30% NH4 OH/EtOH (or n -PrOH) was appropriate eluent for recovering ABX. EDTA added in milk ensured smooth milk flow through CPAC and increased REs . Commercial SPE cartridge, chemical deproteinization and tedious steps were avoided. Abstract: CPAC-SPE-HPLC (coconut powdered activated carbon -SPE- HPLC) has been developed for the determination of antibiotic (ABX), sulfamonomethoxine sodium (SMM), oxytetracycline (OTC), ceftiofur hydrochloride (CEF) and marbofloxacin (MAR), in water and milk. Over 99.0% SMM and OTC were recovered from 20 mL of 0.5 μg/mL ABX solution using 10 mg-CPAC for adsorption and 2 mL of 30% NH4 OH/EtOH (1/19 v/v) for elution. Similarly, over 99.0% CEF and MAR were recovered using 15 mg-CPAC and 2 mL of 30% NH4 OH/ n -PrOH (1/19 v/v). Moreover, the recovery efficiencies of various ABX from 5 to 80 mL of 0.02–2.00 μg/mL medicated milk containing 10 mM EDTA are ordered as follows: OTC (99.3%), SMM (99.1%) > CEF (68.9%) > MAR (61.4%). No interference towards HPLC analysis were observed with elution using 2 mL of 30% NH4 OH/EtOH (1/19 v/v). Furthermore, much lower limit of detections (0.02 μg/mL) than the maximum residual limits from European Commission (0.075–0.100 μg/mL) were obtained.