The effect of Holder pasteurization on the lipid and metabolite composition of human milk. (1st August 2022)

• Record Type: Journal Article
• Authors: Ten-Doménech, Isabel; Ramos-Garcia, Victoria; Moreno-Torres, Marta; Parra-Llorca, Anna; Gormaz, María; Vento, Máximo; Kuligowski, Julia; Quintás, Guillermo
• Is Part Of: Food chemistry. Volume 384(2022)
• Publication Date: 2022-08-01
• Subjects: Donor human milk -- Holder pasteurization -- Preterm infant -- Metabolomics -- Lipidomics -- Network analysis; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132581
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Holder pasteurization (HoP) modifies human milk composition and functionality. HoP provokes alterations at the metabolic pathway level. Lipid enrichment analysis unveils changes on lipid storage and signalling upon HoP. HoP resulted in a median decrease in fatty acid concentration of 10% Future studies should address relevance of alterations for preterm infant nutrition. Abstract: Human milk (HM) is the gold standard for newborn nutrition. When own mother's milk is not sufficiently available, pasteurized donor human milk becomes a valuable alternative. In this study we analyzed the impact of Holder pasteurization (HoP) on the metabolic and lipidomic composition of HM. Metabolomic and lipidomic profiles of twelve paired HM samples were analysed before and after HoP by liquid chromatography–mass spectrometry (MS) and gas chromatography-MS. Lipidomic analysis enabled the annotation of 786 features in HM out of which 289 were significantly altered upon pasteurization. Fatty acid analysis showed a significant decrease of 22 out of 29 detectable fatty acids. The observed changes were associated to five metabolic pathways. Lipid ontology enrichment analysis provided insight into the effect of pasteurization on physical and chemical properties, cellular components, and functions. Future research should focus on nutritional and/or developmental consequences of these changes.