A new amine moiety-based near-infrared fluorescence probe for detection of formaldehyde in real food samples and mice. (1st August 2022)

• Record Type: Journal Article
• Authors: Ding, Ning; Li, Zhao; Hao, Yitong; Yang, Xingbin
• Is Part Of: Food chemistry. Volume 384(2022)
• Publication Date: 2022-08-01
• Subjects: Fluorescent probe -- Formaldehyde -- Paper chips -- Food -- Mice; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132426
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: The designed and synthesized NIR fluorescent probe-NH2 achieved highly sensitive and selective detection of formaldehyde. Paper chips are used to detect formaldehyde through fluorescence signals for the first time. Probe-NH2 was used for the rapid detection of formaldehyde in real food samples. Probe-NH2 has been successfully applied to visualize endogenous formaldehyde in mice. Abstract: A new amine moiety-based near-infrared fluorescent probe (Probe-NH2 ) is developed for detection of formaldehyde in food samples and mice. Probe-NH2 is constructed and synthesized from the IR-780 via two-step reactions as a hemicyanine skeleton bearing an amino moiety. The response mechanism is based on Schiff base reaction that formaldehyde reacts with amine group to form the corresponding imines. Probe-NH2 for detection of formaldehyde exhibits excellent analytical performance, including near-infrared fluorescence emission at 708 nm, high selectivity and sensitivity, also provides a response time as low as 30 min with a detection limit of 1.87 μmolL −1 . Notably, we constructed a simple, rapid and visual formaldehyde detection platform based on paper chips in the near-infrared region for the first time. The accurate detection of formaldehyde in real food samples is of great significance, Probe-NH2 was detected in dried beancurd sticks, endive sprout, frozen shrimp and squid, with good recoveries of 99.60%-112.72%, indicating the reliability of Probe-NH2 for spiked determination of formaldehyde in contaminated foods. More importantly, Probe-NH2 has been successfully applied to the detection of endogenous formaldehyde in mice.