Development and physicochemical characterization of nanoliposomes with incorporated oleocanthal, oleacein, oleuropein and hydroxytyrosol. (1st August 2022)

• Record Type: Journal Article
• Authors: Li, Wenjun; Chountoulesi, Maria; Antoniadi, Lemonia; Angelis, Apostolis; Lei, Jiandu; Halabalaki, Maria; Demetzos, Costas; Mitakou, Sofia; Skaltsounis, Leandros A.; Wang, Chengzhang
• Is Part Of: Food chemistry. Volume 384(2022)
• Publication Date: 2022-08-01
• Subjects: Nanoliposomes -- Oleocanthal -- Oleacein -- Cryo-TEM -- Ritger-Peppas model -- Antioxidant activity; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132470
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Olive bioactive polyphenols were successfully incorporated in nanoliposomes. Physicochemical behavior of liposomes differs depending on the encapsulated compound. In vitro compound release from liposomes is well-fitted with Ritger-Peppas model. Protection of compounds and targeted delivery can be achieved. Abstract: Oleocanthal, oleacein, oleuropein and hydroxytyrosol comprise characteristic polyphenols of olive with high biological value. However, stability problems hinder their further investigation. Thus, in the present study they were incorporated in nanoliposomes by thin film hydration method. The particles sizes, PDI, zeta-potential and physicochemical stabilities of nanoliposomes were evaluated by light scattering methods while FTIR, XRD, TGA and DSC methods were carried out for further physicochemical characterization. Their micromorphology was illustrated by negative-staining TEM and Cryo-TEM, revealing well-dispersed round-shaped vesicles. According to in vitro release studies, oleocanthal and oleacein were rapidly released in a higher percentage than oleuropein and hydroxytyrosol and compatible with the Ritger-Peppas model release mechanism while only oleuropein liposomes were governed by anomalous diffusion of non-Fickian diffusion. Antioxidant assays showed that nanoliposomes presented comparable activity with pure compounds enabling them as suitable carriers for the delivery of olive active biophenols in the human organism.