Detoxification of ochratoxin A and zearalenone by Pleurotus ostreatus during in vitro gastrointestinal digestion. (1st August 2022)

• Record Type: Journal Article
• Authors: Nobre, Clarisse; González, Alondra; Losoya, Carolina; Teixeira, José A.; Belmares, Ruth; Abrunhosa, Luís
• Is Part Of: Food chemistry. Volume 384(2022)
• Publication Date: 2022-08-01
• Subjects: Ochratoxin A -- Zearalenone -- Pleurotus ostreatus -- Biotransformation -- Mycotoxins adsorption -- In vitro gastrointestinal digestion; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132525
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Pleurotus ostreatus powder biotransformed OTA into OTα preferably at pH 5 and 7. Pleurotus ostreatus powder (PO) adsorbed ZEN preferably at pH 3. In the gastrointestinal model, PO eliminated OTA by 95–85% and ZEN by 55–23% The feed matrices reduced mycotoxins bioaccessibility under simulated conditions. With feed matrices, OTA and ZEN were eliminated by up to 34% and 20%, respectively. Abstract: Powdered Pleurotus ostreatus (PO) was tested for the detoxification of ochratoxin A (OTA) and zearalenone (ZEN) through in vitro gastrointestinal digestion in the absence and presence of ground feed and cornmeal. Ochratoxin α was detected in the chromatograms after OTA elimination, suggesting the presence of an OTA-hydrolytic enzyme in the PO, whereas ZEN was adsorbed by PO. OTA was totally eliminated at pH 5 and 7, while ZEN was better adsorbed at pH 3. In simulated gastrointestinal conditions conducted without feed matrices, PO eliminated OTA by 85% and ZEN by 23% at the end of the intestinal phase. When digestions were conducted with ground feed and cornmeal, PO eliminated OTA by 13 and 34%, and ZEN by 20 and 2%, respectively. PO demonstrated great potential for OTA detoxification, but the feed matrices adsorbed the mycotoxins, reducing their bioaccessibility considerably in the oral and gastric phases and negatively influencing PO detoxification capacity.