Profiling of naturally occurring folates in a diverse soybean germplasm by HPLC-MS/MS. (1st August 2022)

• Record Type: Journal Article
• Authors: Agyenim-Boateng, Kwadwo Gyapong; Zhang, Shengrui; Islam, Md Shariful; Gu, Yongzhe; Li, Bin; Azam, Muhammad; Abdelghany, Ahmed M.; Qi, Jie; Ghosh, Suprio; Shaibu, Abdulwahab S.; Gebregziabher, Berhane Sibhatu; Feng, Yue; Li, Jing; Li, Yinghui; Zhang, Chunyi; Qiu, Lijuan; Liu, Zhangxiong; Liang, Qiuju; Sun, Junming
• Is Part Of: Food chemistry. Volume 384(2022)
• Publication Date: 2022-08-01
• Subjects: Soybean (Glycine max L. Merrill) -- Folate -- HPLC-MS/MS -- Germplasm -- Natural variation -- Elite accessions; 10-Formyl-folic acid (PubChem CID: 135405023) -- 5, 10-Methenyl-tetrahydrofolate (PubChem CID: 135398657) -- 5-Formyl-tetrahydrofolate (PubChem CID: 135403648) -- 5-Methyl-tetrahydrofolate (PubChem CID: 135483998) -- Dihydrofolate (PubChem CID: 135398604) -- Folic acid (PubChem CID: 135398658) -- Tetrahydrofolate (PubChem CID: 135444742) -- Methotrexate (PubChem CID: 126941) -- Sodium phosphate monobasic (PubChem CID: 23672064) -- Sodium phosphate dibasic (PubChem CID: 24203) -- Sodium ascorbate (PubChem CID: 23667548) -- β-Mercaptoethanol (PubChem CID: 1567) -- α-Amylase (PubChem CID: 62698) -- Acetonitrile (PubChem CID: 6342) -- Formic acid (PubChem CID: 284); Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2022.132520
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: Conjugase treatment was sufficient for soybean folate extraction. 10-fold variation was observed for soybean folates, from 64.51 − 691.24 μg/100 g. 5-CHO-H4 folate was the most dominant folate vitamer in soybean. Folates are affected by accession type, ecoregion, and seed morphological traits. Soybean is a key candidate for folate biofortification. Abstract: Soybean is a rich source of folates. We optimised the extraction and detection of folates from soybean seeds by HPLC-MS/MS and analysed the folate content and composition of 1074 accessions. Total folate content ranged from 64.51 to 691.24 μg/100 g fresh weight, with 10-fold variation, and 60 elite accessions with over 400 μg/100 g of total folate were identified. The most abundant component was 5-CHO-H4 folate, which accounted for an average of 60% of total folate content. Seed-coat colour, seed weight, ecoregion, and accession type significantly affected soybean folate content. Furthermore, 5-CH3 -H4 folate correlated positively with seed protein ( r = 0.24***) and negatively with oil ( r = -0.26***). The geographical distribution of folate according to accession origin revealed that accessions from Northeast China contain higher amounts of total folate and 5-CHO-H4 folate. This study provides comprehensive and novel insights into the folate profile of soybean, which will benefit soybean breeding for folate enhancement.