An electrochemical aptasensor based on dual-enzymes-driven target recycling strategy for patulin detection in apple juice. (July 2022)

• Record Type: Journal Article
• Authors: Lu, Xia; He, Baoshan; Liang, Ying; Wang, Jinshui; Jiao, Qiang; Liu, Yong; Guo, Rui; Wei, Min; Jin, Huali; Ren, Wenjie; Suo, Zhiguang
• Is Part Of: Food control. Volume 137(2022)
• Publication Date: 2022-07
• Subjects: Patulin -- Exonuclease III -- Mg+-DNAzyme -- FeMOF -- Aptasensor -- Silver-palladium nanoparticles; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2022.108907
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: The presence of patulin (PAT) in fruits poses a threat to human health. Herein, a dual-enzymes-driven target recycling (dual-EATR) strategy-based PAT electrochemical aptasensor was constructed. The branched structure of the labeling material silver-palladium nanoparticles (AgPdNPs) can effectively load thionine (Thi). Gold nanoparticles (AuNPs)/Fe metal-organic frameworks (FeMOF)-PEI-GO with effective catalytic activity can amplify the redox signal of Thi. The presence of PAT could trigger the dual target recycling assisted by Exonuclease Ⅲ and Mg2+-dependent DNAzyme. The dual-EATR strategy gradually accumulates detection signals and can realize ultra-sensitive detection of PAT. With the optimal conditions, the developed aptasensor showed satisfactory performance with the range from 5 × 10 −7 ng mL −1 to 5 ng mL −1 and a low detection limit of 0.217 fg mL −1 . Compared with the chromatographic method, the developed aptasensor has consistent detection results. In addition, the aptasensor exhibited favourable selectivity, reproducibility and long-term stability. In the analysis of real samples, the aptasensor had good accuracy (recovery%, 91.0–103) and high precision (RSD%, 2.69–4.98), and relative error of <13% compared with the spiked concentration. It is proved with 95% of confidence level that the spiked sample tested generated a signal response not significantly different from the true value (p > 0.6). The feasibility of the aptasensor in apple juice samples has been verified, providing an excellent choice for the detection of trace PAT. Highlights: A dual-EATR strategy were proposed for ultra-sensitive PAT detection. A DNA signal probe labeled with AgPdNPs was designed for signal amplification. FeMOF-based composite material to improve conductivity and catalyze redox of Thi. The developed aptasensor exhibited a wide detection range and low detection limit.