Fish allergens of turbot (Scophthalmus maximus) parvalbumin triggers food allergy via inducing maturation of bone marrow derived dendritic cells and driving Th2 immune response. Issue 7 (24th March 2022)

• Record Type: Journal Article
• Authors: Wu, Yeting; Lu, Youyou; Huang, Yuhao; Lin, Hong; Xu, Mengyao; Ahmed, Ishfaq; Chen, Guanzhi; Chen, Yan; Li, Zhenxing
• Is Part Of: Food & function. Volume 13:Issue 7(2022)
• Page Start: 4194
• Page End: 4204
• Publication Date: 2022-03-24
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo04070g
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : A high-efficiency evaluation system for allergen sensitization via index changes. Abstract : Aquatic food allergy has become a key food safety problem and therefore it is urgent to study the mechanism of aquatic food allergy. Turbot parvalbumin (PV) is a major marine food allergen that could cause allergic reactions but the cellular and molecular mechanisms remain to be defined. In this study, we used flow cytometry and ELISA, a coupled co-culture system of dendritic cells and T cells, and revealed that PV could promote the maturation of dendritic cells, mainly by inducing bone marrow-derived dendritic cells (BMDCs) to express MHC II and CD86, and promote the cytokines/chemokines IL-6, IFN-γ, IL-23, and IL-12p70, whereas inhibiting TNF-α expression. Our results suggested that murine BMDCs play a crucial role in the effect of PV on the induction of Th2 responses.