Encapsulation of CALB by nucleotide/metal ions coordination nanoparticles: highly selective catalysis of esterification while poor performance in glycerolysis reaction. (16th September 2021)

• Record Type: Journal Article
• Authors: Chen, Wenyi; Xu, Li; Zhong, Nanjing
• Is Part Of: Journal of the science of food and agriculture. Volume 102:Number 5(2022)
• Page Start: 1812
• Page End: 1822
• Publication Date: 2021-09-16
• Subjects: Candida antarctica lipase B -- coordination polymers -- encapsulated enzyme -- esterification -- glycerolysis; Food -- Periodicals; Agriculture -- Periodicals; 664
• DOI: 10.1002/jsfa.11516
• Languages: English
• ISSNs: 0022-5142

Abstract

Abstract: BACKGROUND: Enzymatic esterification is attracting for particular high‐acid oil deacidification. In this study, Candida antarctica lipase B (CALB) was encapsulated into a series of nucleotide‐hybrid metal coordination polymers (CPs), which were constructed by guanosine 5′‐monophosphate (GMP) and various metals. RESULTS: We here found that, most of the present CPs encapsulated CALB (CALB@CPs) samples were highly selective for esterification while poor in glycerolysis reaction. They exhibited quite poor performance in glycerolysis, with triacylglycerols (TAGs) conversion lower than 5%, despite this considerable enzymatic hydrolysis activities were observed. However, they (most of them) showed good performance in esterification of fatty acids and glycerol for TAG synthesis. In addition, the GMP/Tb (CPs constructed by GMP and Tb 3+ ) encapsulated CALB (CALB@GMP/Tb) transformed over 98% of oleic acid into glycerides in the high‐acid oil deacidification process, and TAG content from 87 to 89% was obtained. Moreover, the CALB@GMP/Tb showed good reusability in the esterification system. CONCLUSION: The present CALB@CPs samples are selective for esterification and suitable for high‐acid oils deacidification. This work provides a new system for enzymatic selectivity improvement study. © 2021 Society of Chemical Industry.