Structure of Cardamine hupingshanensis No. 3 Polysaccharide (CHP‐3) and its Effect on Human Lung Cancer A549 Cells. (13th December 2021)

• Record Type: Journal Article
• Authors: Li, Meidong; Zhang, ZiMu; Luo, Ying; Huang, Xiufang; Luo, Kai
• Is Part Of: Stärke. Volume 74:Number 3/4(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-12-13
• Subjects: Cardamine hupingshanensis -- physiological activity -- polysaccharides -- structure analysis; Starch -- Periodicals; 572.566
• DOI: 10.1002/star.202100119
• Languages: English
• ISSNs: 0038-9056

Abstract

Abstract: Four new water‐soluble polysaccharides has previously been isolated from Cardamine hupingshanensis . In this experiment, the structure of C. hupingshanensis polysaccharide NO. 3 (CHP‐3) is analyzed and its in vitro anti‐tumor activity is assessed. Through structural analysis, CHP‐3 is found to have a molecular weight of 80.64 kDa, and be mainly composed of glucose, galactose, and L ‐arabinose; The backbone of CHP‐3 is mainly composed of →5)‐α‐L‐Araf‐(1→ and →4)‐α‐D‐Glc(p)‐(1→), respectively. By studying its anti‐tumor activity in vitro, it can be concluded that treatment with different concentrations of CHP‐3 exerted diverse effects on A549 cells, with an obvious dose‐dependent relationship. Moreover, after treatment with 12.8 mg mL −1 CHP‐3, significant inhibitory effects are observed on the proliferation, migration, and invasion of A549 cells, indicating that it can be used as an anti‐tumor agent for continued in‐depth research. Abstract : The polysaccharide CHP‐3 is extracted from Cardamine hupingshanensis and the structure of CHP‐3 is analyzed by NMR and other techniques. By studying the inhibition of A549 cell activity, CHP‐3 is found to have a significant inhibitory effect on A549 indicating that it can be used as an anti‐tumor agent for continued in‐depth research.