Comparison of the chemical composition and antioxidant stress ability of polysaccharides from Auricularia auricula under different drying methods. Issue 5 (22nd February 2022)

• Record Type: Journal Article
• Authors: Zhang, Yakun; Shi, Qianwen; Jiang, Wen; Yao, Jing; Zeng, Jiangying; Wang, Weimin; Zhang, Yongjun
• Is Part Of: Food & function. Volume 13:Issue 5(2022)
• Page Start: 2938
• Page End: 2951
• Publication Date: 2022-02-22
• Subjects: Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; Nutrition -- Periodicals; 664.07
• DOI: 10.1039/d1fo03956c
• Languages: English
• ISSNs: 2042-6496

Abstract

Abstract : Auricularia auricula fruiting body-derived polysaccharides (AAPs) were dried using different drying procedures, including hot air-, far infrared-, freeze-, and microwave-drying. Abstract : Auricularia auricula fruiting body-derived polysaccharides (AAPs) were dried using different drying procedures, including hot air-, far infrared-, freeze-, and microwave-drying. The influences of different drying procedures on the chemical compositions and antioxidant activity in vitro and in vivo of AAPs were investigated. The results indicated that freeze-dried AAPs (AAPs-F) possessed the highest uronic acid content (33.53%) and the lowest molecular weight (406.77 kDa). Moreover, AAPs-F exhibited the most potent antioxidant abilities in vitro, including ABTS + and DPPH˙ scavenging abilities, ferric reducing power, and metal ion chelating capacity. Besides, AAPs-F could significantly prolong the lifespan of wild-type C. elegans under oxidative stress induced by H2 O2 and methyl viologen ( p < 0.05) and upregulate the mRNA expression levels of daf-16 (>2.7 fold), sod-3 (>9.2 fold), skn-1 (>4.5 fold) and sir-2.1 (>1.9 fold), and play a significant role in protecting C. elegans against apoptosis ( p < 0.05). Hence, freeze-drying was determined as the preferred procedure for obtaining high-quality AAPs.