Analysis of Fhb1 gene and resistance to Fusarium head blight in 3, 177 diverse wheat accessions. (March 2022)

• Record Type: Journal Article
• Authors: Zheng, Nannan; Li, Guangwei; Zhang, Kunpu; Zheng, Hao; Yang, Jin; Yan, Kunfang; Shi, Cuilan; Su, Zhenqi; Chen, Feng; Wang, Daowen; Zheng, Hongyuan
• Is Part Of: Journal of cereal science. Volume 104(2022)
• Publication Date: 2022-03
• Subjects: Disease resistance -- Fusarium head blight -- Fusarium crown rot -- Fhb1 -- Fusarium graminearum -- Fusarium pseudograminearum -- Wheat germplasm; Grain -- Periodicals; Cereal products -- Periodicals; Céréales -- Périodiques; Produits céréaliers -- Périodiques; Cereal products; Grain; Periodicals; 664.705
• DOI: 10.1016/j.jcs.2021.103387
• Languages: English
• ISSNs: 0733-5210

Abstract

Abstract: Fusarium head blight (FHB), elicited mainly by the fungal pathogen Fusarium graminearum ( Fg ), causes serious damages to global wheat production. Development of resistant cultivars is the most efficient measure for controlling FHB, which requires efficient identification and utilization of the wheat germplasm carrying FHB resistance genes. Because the Fhb1 gene recently cloned from the wheat cultivars Sumai 3 and Wangshuibai, designated here as Fhb1-R, confers type II resistance to FHB, we investigated the presence of Fhb1-R in 3, 177 wheat accessions from different countries of the world. By PCR screening with a functional molecular marker amplifying a part of Fhb1 genomic sequence, Fhb1-R was detected in 33 of the 3, 177 accessions. Through two independent inoculation tests with Fg under greenhouse conditions, eight Fhb1-R carrying lines were found to exhibit relatively high levels of type II resistance to FHB, seven of which were newly identified in this work. Remarkably, a substantial proportion of Fhb1-R carrying accessions were actually susceptible to Fg infection, highlighting the fact that the presence of Fhb1-R is not always associated with type II FHB resistance. Further analysis of representative Fhb1-R carrying accessions led to the finding of one Fhb1-R carrying, but FHB susceptible, wheat accession showing moderate resistance to Fusarium crown rot (FCR), another serious disease caused mainly by Fusarium pseudograminearum ( Fp ). Our work provides insight into the presence of Fhb1-R in a large set of diverse wheat germplasm lines and new genetic resources likely useful for improving wheat resistance to FHB or FCR in the future. Graphical abstract: We have investigated Fhb1 gene in 3, 177 diverse wheat accessions, and identified seven novel Fhb1-R carrying, FHB resistant lines and one new FCR resistant genotype through performing replicated inoculation tests with the fungal pathogens Fusarium graminearum or F. pseudograminearum . Image 1 Highlights: The presence of Fhb1-R was investigated in 3, 177 diverse wheat accessions. Seven Fhb1-R carrying accessions showing resistance to FHB were newly identified. Fhb1-R is not always associated with type II resistance to FHB disease.