Whole-genome sequencing of Cryptococcus podzolicus Y3 and data-independent acquisition-based proteomic analysis during OTA degradation. (June 2022)

• Record Type: Journal Article
• Authors: Wei, Meilin; Dhanasekaran, Solairaj; Godana, Esa Abiso; Yang, Qiya; Sui, Yuan; Zhang, Xiaoyun; Ngolong Ngea, Guillaume Legrand; Zhang, Hongyin
• Is Part Of: Food control. Volume 136(2022)
• Publication Date: 2022-06
• Subjects: Whole-genome sequencing -- Cryptococcus podzolicus Y3 -- Ochratoxin A -- Protein expression -- Data-independent acquisition; Food -- Quality -- Periodicals; Food -- Analysis -- Periodicals; Food handling -- Periodicals; Food industry and trade -- Quality control -- Periodicals; Aliments -- Industrie et commerce -- Qualité -- Contrôle -- Périodiques; Aliments -- Qualité -- Périodiques; Aliments -- Analyse -- Périodiques; Hygiène alimentaire -- Périodiques; Food -- Analysis; Food handling; Food -- Quality; Periodicals; Electronic journals; 664.07
• DOI: 10.1016/j.foodcont.2022.108862
• Languages: English
• ISSNs: 0956-7135

Abstract

Abstract: Cryptococcus podzolicus Y3 is an antagonistic yeast that can control postharvest diseases of crops and mycotoxins, which is potentially valuable for ensuring food safety. Our previous study showed that C. podzolicus Y3 can efficiently degrade ochratoxin A (OTA), one of the harmful mycotoxins in the environment and food. However, the genomic information of C. podzolicus Y3 and its protein expression profile during OTA degradation are not available. In this study, whole-genome sequencing of C. podzolicus Y3 was analyzed. Protein expression during OTA degradation was investigated by the data-independent acquisition (DIA) technique. The genome size of C. podzolicus Y3 was about 29, 181, 249 bp length with 58.79% GC content and identified with 17 scaffolds, 9090 coding regions and 127 non-coding regions. The DIA identified 4315 proteins during OTA degradation, among which 101 proteins were significantly up-regulated and 116 were down-regulated. The differentially expressed proteins were mainly involved in reduced glutathione metabolism, phosphatidylinositol signaling system and mitogen-activated protein kinase signaling pathway, calcium ion homeostasis, endoplasmic reticulum protein processing and fatty acid metabolism. Our results provide the genome information of C. podzolicus Y3 for further functional researches. In addition, this study has revealed the toxicity and stress response mechanism of C. podzolicus Y3 during OTA degradation at the protein level. Highlights: Whole-genome information of antagonism C. podzolicus Y3 was perfectly revealed. OTA treatment significantly changed proteins expression of C. podzolicus Y3. OTA disordered the signal transduction pathway and Ca 2+ homeostasis. OTA disordered the ER protein processing and fatty acid metabolism.