A peptide/maltose-binding protein fusion protein used to replace the traditional antigen for immunological detection of deoxynivalenol in food and feed. (1st December 2018)

• Record Type: Journal Article
• Authors: Xu, Yang; Yang, Hongwei; Huang, Zhibing; Li, Yanping; He, Qinghua; Tu, Zhui; Ji, Yanwei; Ren, Wenjie
• Is Part Of: Food chemistry. Volume 268(2018)
• Page Start: 242
• Page End: 248
• Publication Date: 2018-12-01
• Subjects: Deoxynivalenol -- Mimic epitope peptide -- Enzyme-linked immunosorbent assay -- Immunochromatographic assay; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.06.096
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: A DON antigen mimic (D8 -maltose-binding protein [MBP]) was synthesized. D8 -MBP ELISA and D8 -MBP GICA were then established based on D8 -MBP. The detection threshold of the immunochromatographic assay for D8 -MBP was 25 ng/mL. D8 -MBP could be used to replace the DON-BSA antigen for the detection of DON. Abstract: A deoxynivalenol (DON) epitope clone (D8 ) was obtained by phage display technology using anti-DON monoclonal antibodies as a target molecule. Subsequently, a DON antigen mimic (D8 -maltose-binding protein [MBP]) was synthesized by fusing the mimic epitope peptide with MBP. An enzyme-linked immunosorbent assay (ELISA) and urchin-like gold nanoparticle immunochromatographic assay was developed based on D8 -MBP for detection of DON in maize and wheat. The half-maximal inhibitory concentration, lower detection limit, and linear range of the D8 -MBP ELISA were 57.98 ± 0.97, 9.83, and 11.32–286.77 ng/mL, respectively. The sensitivity of the D8 -MBP ELISA was nearly 2.5 times higher than that of traditional ELISA using DON-bovine serum albumin (BSA). The detection threshold of the colloidal gold immunochromatographic assay for D8 -MBP was 25 ng/mL. Thus, D8 -MBP could be used to replace the traditional DON-BSA antigen for the immunological detection of DON, permitting low cost, rapid detection of DON.