Pharmacokinetics of low molecular weight phenolic compounds in gerbil plasma after the consumption of calafate berry (Berberis microphylla) extract. (1st December 2018)

• Record Type: Journal Article
• Authors: Bustamante, Luis; Pastene, Edgar; Duran-Sandoval, Daniel; Vergara, Carola; Von Baer, Dietrich; Mardones, Claudia
• Is Part Of: Food chemistry. Volume 268(2018)
• Page Start: 347
• Page End: 354
• Publication Date: 2018-12-01
• Subjects: Hydroquinone (PubChem CID: 785) -- Syringic Acid (PubChem CID: 10742) -- Vanillic Acid (PubChem CID: 8468) -- Phenylacetic Acid (PubChem CID: 999) -- 3-Hydroxyphenylacetic Acid (PubChem CID: 12122) -- 3, 4-Dihydroxyphenylacetic Acid (PubChem CID: 547) -- Homovanillic Acid (PubChem CID: 1738) -- p-Coumaric Acid (PubChem CID: 637542) -- m-Coumaric Acid (PubChem CID: 637541); Berberis microphylla -- Bioavailability -- Calafate -- GC–MS -- Pharmacokinetics -- Phenolic acid -- Plasma; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.06.048
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A CBE was prepared containing high concentration of anthocyanins and HCADs. Sixteen phenolic acids increased in gerbil plasma after CBE intake. Concentration of phenylacetic acid derivatives were highest after CBE intake. Main increase of phenolic catabolites was observed after 2 h of CBE intake. A metabolization pathway of phenolics in gerbil plasma after CBE intake is proposed. Abstract: Calafate is a berry with high concentration of anthocyanins and hydroxycinnamic acids that grows in South Patagonia. To date, no metabolism studies of phenolic compounds using calafate have been carried out. A calafate extract was characterized by HPLC-DAD-ESI-MS/MS. After extract administration (300 mg/kg), a pharmacokinetic study of phenolic compounds in gerbil plasma was performed by GC–MS/MS. Sixteen phenolic acids increased after intake. Phenylacetic acid derivatives exhibit the highest concentration, while main increase of phenolic catabolites was observed 2 h post-intake. 3-hydroxyphenylacetic and phenylacetic acids increased at 4–8 h post-intake. All catabolites found in gerbil plasma exhibit concentration peaks between 0.1 and 1 µM, however no parental anthocyanins were detected. Establish in vivo plasmatic concentration ranges of phenolic compounds derived from polyphenol consumption following WHO recommendations, plays a key role to carry out future in vitro assays in order to correctly assign biological benefits of calafate berry consumption.