Effects of soy protein and β-conglycinin on microbiota and invitro antioxidant and immunomodulatory capacities of human faecal cultures. (June 2022)

• Record Type: Journal Article
• Authors: Xia, Yumeng; Kuda, Takashi; Nakamura, Saori; Yamamoto, Mahiro; Takahashi, Hajime; Kimura, Bon
• Is Part Of: Food hydrocolloids. Volume 127(2022)
• Publication Date: 2022-06
• Subjects: Soy protein -- β-Conglycinin -- Human gut microbiota -- Butyrate producers -- Antioxidant capacity -- In vitro immunomodulatory Activity; Hydrocolloids -- Periodicals; Food additives -- Periodicals; Colloïdes -- Périodiques; Aliments -- Additifs -- Périodiques; Colloids; Food additives; Periodicals; Electronic journals; 664.06
• DOI: 10.1016/j.foodhyd.2022.107516
• Languages: English
• ISSNs: 0268-005X

Abstract

Abstract: To clarify the effects of soy protein (SP) and β -conglycinin (BC) on human gut microbiota, human stool samples, collected from four healthy volunteers, were incubated without (control) or with 3% (w/v) SP and BC at 37 °C for 24 h under anaerobic conditions. During incubation, the amount of dissolved protein decreased and that of ammonia increased, although indole content was lower in both SP and BC cultures than in control cultures. The degraded protein band pattern, obtained by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, varied across individuals. Total organic acids tended to increase in amount in SP and BC cultures, with n -butyric acid levels increasing significantly ( p < 0.05) in the BC culture. The gut microbiota were analysed using 16S rRNA (V4) gene amplicon sequencing. Genera regarded as butyrate producers, such as Roseburia, Butyricicoccus, and Faecalibacterium, were high in both SP and BC cultures, and the genera and amplicon sequence variants of butyrate producers varied across individuals. The incubation with SP and BC increased the superoxide anion radical scavenging capacity and ferric reducing power of the faecal cultures. As an in vitro immunomodulatory activity, increased promotion of nitric oxide secretion in murine macrophage-like RAW264.7 cells was observed in the BC cultures. Overall, the effects of SP and BC were considered positive with respect to the host. Graphical abstract: Image 1 Highlights: Human faecal samples were incubated with soy protein (SP) and β- conglycinin (BC). After 24 h incubation, butyrate was high in the BC culture. Butyrate producers Butyricicoccus, Roseburia and Faecalibacterium were high in SP and BC. O2 − scavenging and immunomodulation capacity increased in SP and BC cultures.