Simultaneous determination of clanobutin, dichlorvos, and naftazone in pork, beef, chicken, milk, and egg using liquid chromatography-tandem mass spectrometry. (30th June 2018)

• Record Type: Journal Article
• Authors: Park, Jin-A.; Abd El-Aty, A.M.; Zheng, Weijia; Kim, Seong-Kwan; Cho, Sang-Hyun; Choi, Jeong-min; Hacımüftüo, Ahmet; Jeong, Ji Hoon; Wang, Jing; Shim, Jae-Han; Shin, Ho-Chul
• Is Part Of: Food chemistry. Volume 252(2018)
• Page Start: 40
• Page End: 48
• Publication Date: 2018-06-30
• Subjects: Simultaneous -- Residue analysis -- Determination -- Clanobutin -- Dichlorvos -- Naftazone -- Animal-based food products; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.01.085
• Languages: English
• ISSNs: 0308-8146

Abstract

Graphical abstract: Highlights: Three drugs were quantified in various food products using LC–MS/MS. Samples were extracted using liquid-phase extraction (LPE) or EN-QuEChERS. The LPE efficiency using 0.1% formic acid in acetonitrile was superior to that of EN-QuEChERS with cleanup. The validation parameters were satisfactory for all tested drugs in various matrices. The method can be adopted for various matrices containing protein and fat. Abstract: A chromatographic method involving a single run was validated for the quantification of clanobutin, dichlorvos, and naftazone in products of animal origin. Pork, beef, chicken, milk, and egg samples were extracted with a solution of 0.1% formic acid in acetonitrile, defatted with n -hexane, centrifuged, and filtered prior to analysis using liquid chromatography-tandem mass spectrometry (LC-/MS/MS). The analytes were separated on a C18 column using a solution of 0.1% formic acid and 10 mM ammonium formate (A) and acetonitrile (B) as the mobile phase. A good linearity over 5–50 ng/g concentration range was obtained with coefficients of determination ( R 2 ) ≥ 0.9807. The intra- and interday accuracy (recovery %) calculated from 3 fortification levels (5, 10, and 20 ng/g) were 73.2–108.1% and 71.4–109.8%, and the precisions (expressed relative standard deviations (RSDs)) were 0.9–12.9% and 1.8–10.6%, respectively, for the 3 tested analytes in animal originated foods. The limits of quantification (LOQs) ranged between 0.1 and 1 ng/g, thus enabling the quantification of residual levels below the uniform maximum residue limit (MRL) of 0.01 mg/kg set for compounds having no MRL. The designated methodology was successfully applied to monitor various samples collected from Seoul; the tested analytes were not quantified in any of the market samples. Conclusively, the developed method is simple, sensitive, and accurate, and could be used for the detection of pharmaceuticals in various animal food matrices with variable protein and fat contents.