Detection and identification of furan fatty acids from fish lipids by high-performance liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry. (30th June 2018)

• Record Type: Journal Article
• Authors: Uchida, Hajime; Itabashi, Yutaka; Watanabe, Ryuichi; Matsushima, Ryoji; Oikawa, Hiroshi; Suzuki, Toshiyuki; Hosokawa, Masashi; Tsutsumi, Naonobu; Ura, Kazuhiro; Romanazzi, Donato; Miller, Matthew R.
• Is Part Of: Food chemistry. Volume 252(2018)
• Page Start: 84
• Page End: 91
• Publication Date: 2018-06-30
• Subjects: Furan fatty acids -- High-performance liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry -- Fish lipids -- Oncorhynchus keta -- Macruronus novaezelandiae -- Galeorhinus galeus; Food -- Analysis -- Periodicals; Food -- Composition -- Periodicals; 664
• DOI: 10.1016/j.foodchem.2018.01.044
• Languages: English
• ISSNs: 0308-8146

Abstract

Highlights: A convenient method to screen bioactive furan fatty acids in fish was developed. For this purpose, reversed-phase HPLC/high-resolution ESI-Q-TOF-MS was used. Accurate assignments of furan fatty acids were achieved by measuring exact masses. Clear extracted ion chromatograms were obtained for all furan fatty acid homologs. The method was successfully applied to Japanese and New Zealand fish oil samples. Abstract: Using high-performance liquid chromatography coupled to high-resolution electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC/ESI-Q-TOF-MS), we have developed a new method for detection and identification of furan fatty acids (F-acids), which are widely distributed in living organisms and foods as minor lipid components and are known to have antioxidant and anti-inflammatory effects. For this purpose, total fatty acids prepared from the testis lipids of Japanese chum salmon ( Oncorhynchus keta ) were examined without any concentration or isolation of F-acids. In negative ESI mode, F-acids gave a prominent [M−H] − ion, by which individual F-acids could be detected and identified. High-resolution extracted ion chromatograms clearly showed the occurrence of five major F-acid homologs as already reported by GC/MS. The method was successfully applied to several fish samples and revealed the occurrence of F-acids for the first time in the two New Zealand fish, hoki ( Macruronus novaezelandiae ) and school shark ( Galeorhinus galeus ).